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Genome editing of a rice CDP-DAG synthase confers multipathogen resistance

by Zeng, Qingdong , Zhou, Qi , Li, Yun , Sun, Qiping , Zhang, Wanying , Wang, Yin , Gao, Jie , Zhao, Juan , Xie, Kabin , Jain, Rashmi , ANR-11-INBS-0010, METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation , Gong, Qiuwen , Lawrence Berkeley National Laboratory (LBNL) , Zhou, Wenqing , Kong, Xiaojing , Li, Guotian , Chiu, Tsan-Yu , Qin, Ziting , Han, Xinyu , Chen, Xiaoyang , Xu, Jiandi , Ronald, Pamela, C , Kang, Zhensheng , Boutté, Yohann , Sun, Peng , Zhou, Yaru , Huang, Renliang , Li, Qiang , Plateforme Bordeaux Metabolome , Yang, Lei , Fouillen, Laetitia , Mortimer, Jenny, C , Laboratoire de biogenèse membranaire (LBM) , Guo, Liang , Zheng, Lu , Sha, Gan , MetaboHUB-MetaboHUB

in 38/1 / 38/23 / 38/39 / 45 / 45/44 / 45/70 / 631/1647/1511 / 631/449/2169 / 631/449/2661/2666 / 631/449/448/2024 / 631/61/447/8 / 96/35 / 96/63 / Acids / Agricultural production / Alleles / Cellular structure / Diacylglycerol Cholinephosphotransferase - genetics / Diacylglycerol Cholinephosphotransferase - metabolism / Diglycerides / Disease resistance / Disease Resistance - genetics / Editing / Environmental Sciences / Fungal infections / Gene deletion / Gene Editing - methods / Genes / Genome editing / Genome, Plant - genetics / Genomes / Genotype & phenotype / Humanities and Social Sciences / Life Sciences / Medical research / multidisciplinary / Mutation / Oryza - enzymology / Oryza - genetics / Oryza - microbiology / Oryza sativa / Pathogens / Phosphatidylinositol 4,5-diphosphate / Phosphatidylinositol 4,5-Diphosphate - metabolism / Phosphatidylinositols - metabolism / Phospholipids / Plant breeding / Plant Breeding - methods / Plant diseases / Plant Diseases - genetics / Plant Diseases - microbiology / Rice / Science / Science & Technology - Other Topics / Science (multidisciplinary) / Yeast

2023

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2023

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2023

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Journal Article

Genome editing of a rice CDP-DAG synthase confers multipathogen resistance

Zeng, Qingdong,

Zhou, Qi,

Li, Yun,

Sun, Qiping,

Zhang, Wanying,

Wang, Yin,

Gao, Jie,

Zhao, Juan,

Xie, Kabin,

Jain, Rashmi,

ANR-11-INBS-0010, METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation,

Gong, Qiuwen,

Lawrence Berkeley National Laboratory (LBNL),

Zhou, Wenqing,

Kong, Xiaojing,

Li, Guotian,

Chiu, Tsan-Yu,

Qin, Ziting,

Han, Xinyu,

Chen, Xiaoyang,

Xu, Jiandi,

Ronald, Pamela, C,

Kang, Zhensheng,

Boutté, Yohann,

Sun, Peng,

Zhou, Yaru,

Huang, Renliang,

Li, Qiang,

Plateforme Bordeaux Metabolome,

Yang, Lei,

Fouillen, Laetitia,

Mortimer, Jenny, C,

Laboratoire de biogenèse membranaire (LBM),

Guo, Liang,

Zheng, Lu,

Sha, Gan,

MetaboHUB-MetaboHUB

2023

Overview

The discovery and application of genome editing introduced a new era of plant breeding by giving researchers efficient tools for the precise engineering of crop genomes1. Here we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice (Oryza sativa). We first isolated a lesion mimic mutant (LMM) from a mutagenized rice population. We then demonstrated that a 29-base-pair deletion in a gene we named RESISTANCE TO BLAST1 (RBL1) caused broad-spectrum disease resistance and showed that this mutation caused an approximately 20-fold reduction in yield. RBL1 encodes a cytidine diphosphate diacylglycerol synthase that is required for phospholipid biosynthesis2. Mutation of RBL1 results in reduced levels of phosphatidylinositol and its derivative phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). In rice, PtdIns(4,5)P2 is enriched in cellular structures that are specifically associated with effector secretion and fungal infection, suggesting that it has a role as a disease-susceptibility factor3. By using targeted genome editing, we obtained an allele of RBL1, named RBL1Δ12, which confers broad-spectrum disease resistance but does not decrease yield in a model rice variety, as assessed in small-scale field trials. Our study has demonstrated the benefits of editing an LMM gene, a strategy relevant to diverse LMM genes and crops.

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Publisher

Nature Publishing Group,CCSD,Nature Publishing Group UK

Subject

38/1

/ 38/23

/ 38/39

/ 45

/ 45/44

/ 45/70

/ 631/1647/1511