Asset Details
Do you wish to reserve the book?
Bile cell-free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Bile cell-free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer
Do you wish to request the book?
Bile cell-free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Bile cell-free DNA as a novel and powerful liquid biopsy for detecting somatic variants in biliary tract cancer
2019
Overview
Tissue sampling of biliary tract carcinomas (BTCs) for molecular characterization is challenging. The aim of this study was to investigate the possibility of identifying individual actionable mutations derived from bile cell-free DNA (cfDNA) using targeted deep sequencing. Ten BTC patients, four with gallbladder carcinomas and six with cholangiocarcinomas, were enrolled in the present study. Using targeted deep sequencing with a panel of 150 tumor-related genes, paired bile cfDNA and tumor DNA were analyzed for mutational variants individually and then compared. The present study, to the best of our knowledge, is the first to reveal that bile cfDNA is predominantly comprised of long DNA fragments, which is not the case for plasma cfDNA. Herein, paired bile cfDNA and tumors from ten BTC patients were examined using targeted deep sequencing. When comparing bile cfDNA and tumor DNA for single nucleotide variation (SNV)/insertion and deletion (Indel), the results using targeted deep sequencing revealed high sensitivity (94.7%) and specificity (99.9%). Additionally, the sensitivity of detecting a copy number variation (CNV) was 75.0%, with a specificity of 98.9%. When comparing two bile extraction methods, including percutaneous transhepatic cholangial drainage and operation, no significant difference in SNV/Indel or CNV detection sensitivity was noted. Moreover, when examining the tumor stage and incidence site, AJCC stage II and the distal bile duct both had significantly decreased CNV detection sensitivities. The present study revealed that targeted deep sequencing can reliably detect mutational variants within bile cfDNA obtained from BTC patients. These preliminary results may shed light on bile cfDNA as a promising liquid biopsy for BTC patients.
Publisher
D.A. Spandidos,Spandidos Publications,Spandidos Publications UK Ltd
Subject
/ Aged
/ Analysis
/ Antigens
/ Bile
/ Biliary Tract Neoplasms - classification
/ Biliary Tract Neoplasms - diagnosis
/ Biliary Tract Neoplasms - genetics
/ Biomarkers, Tumor - genetics
/ Cancer
/ Cell-Free Nucleic Acids - analysis
/ Cell-Free Nucleic Acids - genetics
/ DNA
/ Female
/ Genes
/ High-Throughput Nucleotide Sequencing
/ Humans
/ Male
/ Mutation
/ Patients
/ Tumors
