Journal Article

Effects of early-life antibiotics on the developing infant gut microbiome and resistome: a randomized trial

Reyman, Marta, Bogaert, Debby, Plötz, Frans B., Willems, Rob J. L., de Waal, Wouter J., van Schaik, Willem, Chu, Mei Ling J. N., van Houten, Marlies A., Schiering, Irene, Watson, Rebecca L., Arp, Kayleigh, Sanders, Elisabeth A. M.

2022

Overview

Broad-spectrum antibiotics for suspected early-onset neonatal sepsis (sEONS) may have pronounced effects on gut microbiome development and selection of antimicrobial resistance when administered in the first week of life, during the assembly phase of the neonatal microbiome. Here, 147 infants born at ≥36 weeks of gestational age, requiring broad-spectrum antibiotics for treatment of sEONS in their first week of life were randomized 1:1:1 to receive three commonly prescribed intravenous antibiotic combinations, namely penicillin + gentamicin, co-amoxiclav + gentamicin or amoxicillin + cefotaxime (ZEBRA study, Trial Register NL4882). Average antibiotic treatment duration was 48 hours. A subset of 80 non-antibiotic treated infants from a healthy birth cohort served as controls (MUIS study, Trial Register NL3821). Rectal swabs and/or faeces were collected before and immediately after treatment, and at 1, 4 and 12 months of life. Microbiota were characterized by 16S rRNA-based sequencing and a panel of 31 antimicrobial resistance genes was tested using targeted qPCR. Confirmatory shotgun metagenomic sequencing was executed on a subset of samples. The overall gut microbial community composition and antimicrobial resistance gene profile majorly shift directly following treatment (R 2  = 9.5%, adjusted p -value = 0.001 and R 2  = 7.5%, adjusted p -value = 0.001, respectively) and normalize over 12 months (R 2  = 1.1%, adjusted p -value = 0.03 and R 2  = 0.6%, adjusted p -value = 0.23, respectively). We find a decreased abundance of Bifidobacterium spp. and increased abundance of Klebsiella and Enterococcus spp. in the antibiotic treated infants compared to controls. Amoxicillin + cefotaxime shows the largest effects on both microbial community composition and antimicrobial resistance gene profile, whereas penicillin + gentamicin exhibits the least effects. These data suggest that the choice of empirical antibiotics is relevant for adverse ecological side-effects. Here, in a randomized trial of 147 infants receiving distinct antibiotic regimens for early-onset neonatal sepsis, Reyman et al. characterize the gut microbiome and resistance profiles, finding differential effects of antibiotic combinations on microbial community composition and antimicrobial resistance genes.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 45/23 / 45/62 / 45/77 / 631/326/22/1290 / 631/326/22/1434 / 631/326/2565/2134 / 631/326/41/2142 / 692/700/1720/3192 / Abundance / Age / Amoxicillin / Amoxicillin-Potassium Clavulanate Combination - pharmacology / Anti-Bacterial Agents - adverse effects / Anti-Bacterial Agents - pharmacology / Antibiotics / Antimicrobial agents / Antimicrobial resistance / Bacteria - classification / Bacteria - isolation & purification / Bifidobacterium - isolation & purification / Cefotaxime / Cefotaxime - pharmacology / Community composition / Digestive system / Drug resistance / Ecological effects / Enterococcus - isolation & purification / Gastrointestinal Microbiome - drug effects / Gastrointestinal Microbiome - genetics / Genes / Gentamicin / Gentamicins - pharmacology / Gestational age / Gut microbiota / Humanities and Social Sciences / Humans / Infant, Newborn / Infants / Intestinal microflora / Intravenous administration / Klebsiella / Klebsiella - isolation & purification / Metagenomics / Microbial Sensitivity Tests / Microbiomes / Microbiota / Microorganisms / multidisciplinary / Neonatal Sepsis - drug therapy / Neonates / Penicillin / Penicillins - pharmacology / RNA, Ribosomal, 16S - genetics / rRNA 16S / Science / Science (multidisciplinary) / Sepsis