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Changes in rumen bacterial community composition in steers in response to dietary nitrate
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Changes in rumen bacterial community composition in steers in response to dietary nitrate
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Journal Article
Changes in rumen bacterial community composition in steers in response to dietary nitrate
2013
Overview
The effect of dietary nitrate supplementation on rumen bacterial community composition was examined in beef steers fed either a nitrate-N diet or urea-N diet. An automated method of ribosomal intergenic spacer analysis was applied to solid and liquid fractions of ruminal contents to allow comparison of bacterial communities. Supplemental N source affected relative population size of four amplicon lengths (ALs) in the liquid fraction and three ALs in the solid fraction. Five ALs were more prevalent after adaptation to nitrate. Correspondence analysis indicated that feeding the steers the nitrate-N diet versus urea-N diet changed the bacterial community composition in the liquid but not in the solid fraction. This led to an investigation of the relative sizes of potential nitrate-reducing populations.
Mannheimia succiniciproducens
,
Veillonella parvula
, and
Campylobacter fetus
were obtained from nitrate enrichment culture and quantified by real-time PCR based on 16S rRNA sequence. Nitrate supplementation increased the percentage of
C
.
fetus
in the liquid and solid phases, and in solid phase, the percentage of
M
.
succiniciproducens
increased. No change in species prevalence was observed for
V
.
parvula
. However, even after adaptation to dietary nitrate, the relative population sizes for all three putative nitrate-reducing species were very low (<0.06 % of 16S rRNA gene copy number). The liquid-associated bacterial community composition changed due to nitrate supplementation, and at least part of this change reflects an increase in the species prevalence of
C
.
fetus
, a species which is not typically regarded as a ruminal inhabitant.
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Analysis
/ Animals
/ Applied Microbial and Cell Physiology
/ Bacteria
/ Biomedical and Life Sciences
/ Biota
/ Campylobacter fetus - genetics
/ Campylobacter fetus - isolation & purification
/ Cattle
/ Diet
/ DNA, Ribosomal Spacer - chemistry
/ DNA, Ribosomal Spacer - genetics
/ Feeds
/ Fetuses
/ Microbial Genetics and Genomics
/ Nitrates
/ Nitrates - administration & dosage
/ Pasteurellaceae - isolation & purification
/ Proteins
/ RNA, Ribosomal, 16S - genetics
/ rRNA 16S
/ Rumen
/ Studies
/ Urea
