Journal Article

Deciphering the complex role of thrombospondin-1 in glioblastoma development

Dedieu, Stéphane, Pineau, Raphael, Feige, Jean-Jacques, Falciani, Francesco, Salabert, Laura, Daubon, Thomas, Bello, Lorenzo, Miletic, Hrvoje, Guérit, Sylvaine, Bailly, Sabine, Andrique, Laetitia, Léon, Céline, Darbo, Elodie, Bikfalvi, Andréas, Maitre, Marlène, Rossi, Marco, Jeanne, Albin, Clarke, Kim, Bjerkvig, Rolf

2019

Overview

We undertook a systematic study focused on the matricellular protein Thrombospondin-1 (THBS1) to uncover molecular mechanisms underlying the role of THBS1 in glioblastoma (GBM) development. THBS1 was found to be increased with glioma grades. Mechanistically, we show that the TGFβ canonical pathway transcriptionally regulates THBS1, through SMAD3 binding to the THBS1 gene promoter. THBS1 silencing inhibits tumour cell invasion and growth, alone and in combination with anti-angiogenic therapy. Specific inhibition of the THBS1/CD47 interaction using an antagonist peptide decreases cell invasion. This is confirmed by CD47 knock-down experiments. RNA sequencing of patient-derived xenograft tissue from laser capture micro-dissected peripheral and central tumour areas demonstrates that THBS1 is one of the gene with the highest connectivity at the tumour borders. All in all, these data show that TGFβ1 induces THBS1 expression via Smad3 which contributes to the invasive behaviour during GBM expansion. Furthermore, tumour cell-bound CD47 is implicated in this process. Thrombospondin-1 (THSB1) is a component of the ECM with a role in regulating cancer development and tumour vasculature. Here, the authors show that TGF-beta-induced THBS1 expression contributes to the invasive behaviour of GBM cells and promotes resistance to antiangiogenic therapy partially through interaction with CD47.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 13 / 13/1 / 13/106 / 13/109 / 13/51 / 45 / 45/91 / 631/67/1246 / 631/80/474/2280 / 64/60 / Angiogenesis / Animals / Brain cancer / Brain Neoplasms - blood supply / Brain Neoplasms - genetics / Brain Neoplasms - mortality / Brain Neoplasms - pathology / Cancer / CD47 Antigen - antagonists & inhibitors / CD47 Antigen - genetics / CD47 Antigen - metabolism / Cell Line, Tumor / Cerebral Cortex / Gene expression / Gene Expression Regulation, Neoplastic / Gene sequencing / Glioblastoma / Glioblastoma - blood supply / Glioblastoma - genetics / Glioblastoma - mortality / Glioblastoma - pathology / Glioma / Humanities and Social Sciences / Humans / Laser Capture Microdissection / Life Sciences / Male / Medical prognosis / Mice / Mice, Knockout / Molecular modelling / multidisciplinary / Neoplasm Invasiveness / Oncology / Peptides / Peptides - pharmacology / Promoter Regions, Genetic / Protein Binding / Proteins / Ribonucleic acid / RNA / RNA, Small Interfering - genetics / RNA, Small Interfering - metabolism / Science / Science (multidisciplinary) / Signal Transduction / Smad3 protein / Smad3 Protein - genetics / Smad3 Protein - metabolism / Spheroids, Cellular - metabolism / Spheroids, Cellular - pathology / Survival Analysis / Thrombospondin / Thrombospondin 1 - antagonists & inhibitors / Thrombospondin 1 - genetics / Thrombospondin 1 - metabolism / Transcription / Transforming Growth Factor beta1 - genetics / Transforming Growth Factor beta1 - metabolism / Transforming growth factor-b1 / Tumors / Xenograft Model Antitumor Assays / Xenografts / Xenotransplantation