Journal Article

High expression of CDCA7 predicts poor prognosis for clear cell renal cell carcinoma and explores its associations with immunity

Wang, Yi, Wang, Zengjun, Miao, Chenkui, Xing, Qianwei, Liu, Shouyong

2021

Overview

Background Cell division cycle-associated 7 (CDCA7), as a member of the cell division cycle associated family, was reported to be aberrantly expressed in both solid tumors and hematological tumors, suggesting its essential role in promoting tumorigenesis. Hence, we aimed to explore its comprehensive roles of overall survival (OS) in clear cell renal cell carcinoma (ccRCC) and emphasize its associations with immunity. Methods The RNA sequencing data and corresponding clinical information were downloaded from The Cancer Genome Atlas (TCGA) database. Gene set enrichment analysis (GSEA) was adopted to explore CDCA7 associated signaling pathways. Univariate and multivariate Cox regression analyses were carried out to assess independent prognostic factors. Furthermore, roles of CDCA7 in human immunity were also investigated. Results Our results suggested that CDCA7 was overexpressed in ccRCC and its elevated expression was related to shorter OS (P < 0.01). Univariate and multivariate Cox regression analyses identified CDCA7 as an independent prognostic factor (both P < 0.05). The prognostic nomogram integrating CDCA7 expression level and clinicopathologic variables was constructed to predict 1-, 3- and 5-year OS. GSEA indicated that high CDCA7 expression was related to the apoptosis pathway, cell cycle pathway, JAK-STAT pathway, NOD like receptor pathway, P53 pathway, T cell receptor pathway and toll like receptor pathway, etc. Moreover, CDCA7 was significantly related to microsatellite instability (MSI, P < 0.001) and tumor mutational burden (TMB, P < 0.001). As for immunity, CDCA7 was remarkably associated with immune infiltration, tumor microenvironment, immune checkpoint molecules and immune pathways. Conclusions CDCA7 could serve as an independent prognostic factor for ccRCC and it was closely related to MSI, TMB, and immunity.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Apoptosis / Biomedical and Life Sciences / Biomedicine / Breast cancer / Cancer Research / CDCA7 / Cell Biology / Cell cycle / Cell division / Cell survival / Clear cell renal cell carcinoma / Clear cell-type renal cell carcinoma / Correlation analysis / Datasets / Ethnicity / Gender / Gene expression / Gene set enrichment analysis / Genomes / Immune checkpoint / Immunity / Kidney cancer / Lymphocytes T / Medical prognosis / Metastases / Microsatellite instability / Mutation / Nomograms / p53 Protein / Performance evaluation / Phase transitions / Primary Research / Prognosis / Proteins / Regression analysis / Software / Solid tumors / Survival / Survival analysis / T cell receptors / Toll-like receptors / Tumor microenvironment / Tumorigenesis / Tumors