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SETD8 inhibits ferroptosis in pancreatic cancer by inhibiting the expression of RRAD
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SETD8 inhibits ferroptosis in pancreatic cancer by inhibiting the expression of RRAD
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Journal Article
SETD8 inhibits ferroptosis in pancreatic cancer by inhibiting the expression of RRAD
2023
Overview
Background
As an oncogene,
SETD8
can promote tumour growth and tumour cell proliferation. This study aims to reveal the relationship between
SETD8
and ferroptosis in pancreatic cancer and its role in pancreatic cancer to provide a possible new direction for the comprehensive treatment of pancreatic cancer.
Methods
The downstream targets were screened by RNA sequencing analysis. Western blot, Real-time Quantitative PCR (qPCR) and immunohistochemistry showed the relationship between genes. Cell proliferation analysis and cell metabolite analysis revealed the function of genes. Chromatin immunoprecipitation (CHIP) assays were used to study the molecular mechanism.
Results
The potential downstream target of
SETD8
,
RRAD
, was screened by RNA sequencing analysis. A negative correlation between
SETD8
and
RRAD
was found by protein imprinting, Real-time Quantitative PCR (qPCR) and immunohistochemistry. Through cell proliferation analysis and cell metabolite analysis, it was found that
RRAD
can not only inhibit the proliferation of cancer cells but also improve the level of lipid peroxidation of cancer cells. At the same time, chromatin immunoprecipitation analysis (CHIP) was used to explore the molecular mechanism by which
SETD8
regulates
RRAD
expression.
SETD8
inhibited
RRAD
expression.
Conclusions
SETD8
interacts with the promoter region of
RRAD
, which epigenetically silences the expression of
RRAD
to reduce the level of lipid peroxidation in pancreatic cancer cells, thereby inhibiting ferroptosis in pancreatic cancer cells and resulting in poor prognosis of pancreatic cancer.
