Journal Article

DWARF 53 acts as a repressor of strigolactone signalling in rice

Yi, Wei, Li, Jiayang, Xiong, Guosheng, Jiang, Liang, Ma, Honglei, Liu, Huihui, Melcher, Karsten, Xu, H. Eric, Meng, Xiangbing, Liu, Guifu, Yu, Hong, He, Yuanzheng, Wu, Zhongshan, Yuan, Yundong, Qian, Qian, Liu, Xue, Chen, Fulu, Wang, Lei, Zhao, Lihua, Wang, Yonghong

2013

Overview

Strigolactones (SLs) are a group of newly identified plant hormones that control plant shoot branching. SL signalling requires the hormone-dependent interaction of DWARF 14 (D14), a probable candidate SL receptor, with DWARF 3 (D3), an F-box component of the Skp–Cullin–F-box (SCF) E3 ubiquitin ligase complex. Here we report the characterization of a dominant SL-insensitive rice ( Oryza sativa ) mutant dwarf 53 ( d53 ) and the cloning of D53 , which encodes a substrate of the SCF D3 ubiquitination complex and functions as a repressor of SL signalling. Treatments with GR24, a synthetic SL analogue, cause D53 degradation via the proteasome in a manner that requires D14 and the SCF D3 ubiquitin ligase, whereas the dominant form of D53 is resistant to SL-mediated degradation. Moreover, D53 can interact with transcriptional co-repressors known as TOPLESS-RELATED PROTEINS. Our results suggest a model of SL signalling that involves SL-dependent degradation of the D53 repressor mediated by the D14–D3 complex. Strigolactones (SLs), key regulators of plant growth, are believed to mediate their responses through a proposed receptor (D14) that interacts with an F-box protein (D3) to form a D14–SCF D3 protein complex; here the perception of SLs by the D14–SCF D3 complex and the control of gene expression are linked by the finding that DWARF 53, a repressor protein of SL signalling, interacts with the D14–SCF D3 complex and is ubiquitinated and degraded in a SL-dependent manner. Strigolactone receptor identified The strigolactones are key regulators of plant growth, controlling the formation of secondary shoots and regulating root branching. Strigolactone responses are mediated through a proposed receptor (D14) that interacts with an F-box protein (D3). Now, in two related publications, Liang Jiang et al . and Feng Zhou et al . demonstrate a functional link between the perception of strigolactones by D14/D3 and the control of gene expression in rice. They show that the protein DWARF53 (D53), of previously unknown function, acts as a repressor of strigolactone signalling and that strigolactones induce its degradation. D53 interacts with the D14–D3 complex and is ubiquitinated and degraded by the proteasome in a strigolactone-dependent manner.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13/1 / 13/106 / 13/109 / 13/44 / 13/95 / 14/19 / 14/34 / 14/35 / 38/77 / 631/449/1741/2672 / 82 / 82/16 / 82/29 / 82/80 / 82/83 / Agronomy. Soil science and plant productions / Biological and medical sciences / Biosynthesis / Botanical research / Cellular signal transduction / Cloning / Cloning, Molecular / Fundamental and applied biological sciences. Psychology / Gene expression / Gene Expression Regulation, Plant / Genes / Genetic aspects / Genetics and breeding of economic plants / Genotype & phenotype / Hormone receptors / Hormones / Humanities and Social Sciences / Lactones - antagonists & inhibitors / Lactones - metabolism / Models, Biological / multidisciplinary / Multiprotein Complexes - chemistry / Multiprotein Complexes - metabolism / Mutation / Mutation - genetics / Oryza - genetics / Oryza - metabolism / Physiological aspects / Plant Growth Regulators - antagonists & inhibitors / Plant Growth Regulators - metabolism / Plant hormones / Plant Proteins - chemistry / Plant Proteins - genetics / Plant Proteins - metabolism / Proteasome Endopeptidase Complex - metabolism / Protein Binding / Proteins / Proteolysis / Rice / Science / Signal Transduction / Ubiquitin - metabolism