Journal Article

Double blind microarray-based polysaccharide profiling enables parallel identification of uncharacterized polysaccharides and carbohydrate-binding proteins with unknown specificities

Matard-Mann, Maria, Duffieux, Delphine, Pedersen, Henriette L., Czjzek, Mirjam, Salmeán, Armando A., Jam, Murielle, Larocque, Robert, Michel, Gurvan, Guillouzo, Alexia, Hervé, Cécile, Willats, William G. T.

2018

Overview

Marine algae are one of the largest sources of carbon on the planet. The microbial degradation of algal polysaccharides to their constitutive sugars is a cornerstone in the global carbon cycle in oceans. Marine polysaccharides are highly complex and heterogeneous, and poorly understood. This is also true for marine microbial proteins that specifically degrade these substrates and when characterized, they are frequently ascribed to new protein families. Marine (meta)genomic datasets contain large numbers of genes with functions putatively assigned to carbohydrate processing, but for which empirical biochemical activity is lacking. There is a paucity of knowledge on both sides of this protein/carbohydrate relationship. Addressing this ‘double blind’ problem requires high throughput strategies that allow large scale screening of protein activities, and polysaccharide occurrence. Glycan microarrays, in particular the Comprehensive Microarray Polymer Profiling (CoMPP) method, are powerful in screening large collections of glycans and we described the integration of this technology to a medium throughput protein expression system focused on marine genes. This methodology (Double Blind CoMPP or DB-CoMPP) enables us to characterize novel polysaccharide-binding proteins and to relate their ligands to algal clades. This data further indicate the potential of the DB-CoMPP technique to accommodate samples of all biological sources.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/449/2669 / 631/45/72/1205 / 704/829/827 / 82/80 / Algae / Aquatic Organisms - chemistry / Biochemistry / Biochemistry, Molecular Biology / Carbohydrates / Carbon cycle / Carbon sources / Chlorophyta - chemistry / Data collection / Double-blind studies / Environmental Engineering / Environmental Sciences / Escherichia coli / Glycomics - methods / Humanities and Social Sciences / Life Sciences / Microarray Analysis - methods / Microbial degradation / multidisciplinary / Oceans / Phaeophyceae - chemistry / Plants - chemistry / Polymers / Polysaccharides / Polysaccharides - analysis / Protein families / Proteins / Receptors, Cell Surface - analysis / Rhodophyta - chemistry / Saccharides / Science / Science (multidisciplinary)