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Development of an in-house indirect ELISA kit for the serological detection of antibodies against infectious bursal disease in chickens
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Development of an in-house indirect ELISA kit for the serological detection of antibodies against infectious bursal disease in chickens
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Development of an in-house indirect ELISA kit for the serological detection of antibodies against infectious bursal disease in chickens
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Journal Article
Development of an in-house indirect ELISA kit for the serological detection of antibodies against infectious bursal disease in chickens
2025
Overview
Infectious bursal disease is a highly contagious disease of young chickens caused by the infectious bursal disease virus. This disease poses an important threat to the commercial poultry industry globally. This study was designed to develop an In-House Indirect Enzyme-Linked Immune Sorbent Assay Kit for the serological detection of antibodies against infectious bursal disease viruses. An infectious bursal disease virus antigen dilution (1:2), sample serum (1:500), and mouse anti-chicken immunoglobulin G (IgG) labeled with horseradish peroxidase (HRP) (1:2,000) were used in this assay. The calculated cutoff value was 0.24. This in-house indirect ELISA method was compared with a commercial ELISA kit for the detection of antibodies against infectious bursal disease virus in chickens. The performance of the newly developed and commercial ELISA kit was evaluated as described by Samad et al. (1994). The sensitivity and specificity of the current ELISA method were 98% (95% CI: 92.96–99.76) and 97% (95% CI: 91.48–99.38), respectively. The average intra-assay % CV of the triplet of 2 samples was 7.6, and interassay comparisons indicated a CV of 5.45%. As indicated by the results, we described a valuable and cost-effective, sensitive and specific in-house indirect ELISA kit for the serological diagnosis of infectious bursal disease in Ethiopia.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Antibody
/ Antigens
/ Birnaviridae Infections - diagnosis
/ Birnaviridae Infections - immunology
/ Birnaviridae Infections - veterinary
/ Birnaviridae Infections - virology
/ Chicken
/ Chickens
/ Diseases
/ Enzyme-linked immunosorbent assay
/ Enzyme-Linked Immunosorbent Assay - methods
/ Enzyme-Linked Immunosorbent Assay - veterinary
/ Enzymes
/ Ethiopia
/ Identification and classification
/ In-house indirect enzyme-linked immunosorbent assay
/ Infectious bursal disease virus
/ Infectious bursal disease virus - immunology
/ Medicine
/ Poultry
/ Poultry Diseases - diagnosis
/ Poultry Diseases - immunology
/ sorbents
/ Vaccines
/ Veterinary Medicine/Veterinary Science
/ Viruses
/ Zoology
