Journal Article

The root knot nematode resistance gene Mi from tomato is a member of the leucine zipper, nucleotide binding, leucine-rich repeat family of plant genes

Bodeau, J, Zabel, P, Williamson, V.M, Milligan, S.B. (University of California, Davis, CA.), Yaghoobi, J, Kaloshian, I

1998

Overview

The Mi locus of tomato confers resistance to root knot nematodes. Tomato DNA spanning the locus was isolated as bacterial artificial chromosome clones, and 52 kb of contiguous DNA was sequenced. Three open reading frames were identified with similarity to cloned plant disease resistance genes. Two of them, Mi-1.1 and Mi-1.2, appear to be intact genes; the third is a pseudogene. A 4-kb mRNA hybridizing with these genes is present in tomato roots. Complementation studies using cloned copies of Mi-1.1 and Mi-1.2 indicated that Mi-1.2, but not Mi-1.1, is sufficient to confer resistance to a susceptible tomato line with the progeny of transformants segregating for resistance. The cloned gene most similar to Mi-1.2 is Prf, a tomato gene required for resistance to Pseudomonas syringae. Prf and Mi-1.2 share several structural motifs, including a nucleotide binding site and a leucine-rich repeat region, that are characteristic of a family of plant proteins, including several that are required for resistance against viruses, bacteria, fungi, and now, nematodes

• Publisher: American Society of Plant Physiologists
• Subject: ADN / Amino Acid Sequence / AMINO ACID SEQUENCES / Amino acids / Animals / ARN MENSAJERO / ARN MESSAGER / BACTERIAL ARTIFICIAL CHROMOSOMES / Base Sequence / BINDING PROTEINS / CHEMICAL COMPOSITION / chemistry / CHROMOSOME / CHROMOSOMES / Cloning, Molecular / Complementary DNA / COMPLEMENTATION / COMPOSICION QUIMICA / COMPOSITION CHIMIQUE / CROMOSOMAS / DESCENDANCE / DNA / DNA Primers / DNA-binding proteins / DNA-Binding Proteins - genetics / EPS / EXPRESION GENICA / EXPRESSION DES GENES / Gels / GENBANK/AF039631 / GENBANK/AF039682 / GENE / GENE EXPRESSION / GENES / Genes, Plant / genetic complementation / GENETIC RESISTANCE / GENETICA / GENETICS / GENETIQUE / Laboratorium voor Moleculaire biologie / Laboratory of Molecular Biology / LEUCINA / LEUCINE / Leucine - chemistry / Leucine Zippers / Leucine Zippers - genetics / LOCI / LOCUS / LYCOPERSICON ESCULENTUM / Lycopersicon esculentum - genetics / Meloidogyne / MELOIDOGYNE JAVANICA / MESSENGER RNA / metabolism / Microphthalmia-Associated Transcription Factor / MOLECULAR SEQUENCE DATA / Nematoda / Nematoda - pathogenicity / Nematodes / NUCLEOTIDE SEQUENCE / Nucleotides / Nucleotides - metabolism / OPEN READING FRAMES / pathogenicity / PEST RESISTANCE / Plant cells / PLANTAS TRANSGENICAS / PLANTE TRANSGENIQUE / Plants / Plants, Genetically Modified / PROGENIE / PROGENY / PROTEINAS AGLUTINANTES / PROTEINE DE LIAISON / Pseudomonas syringae / RACINE / RAICES / RESISTANCE AUX ORGANISMES NUISIBLES / RESISTANCE GENETIQUE / RESISTENCIA A LAS PLAGAS / RESISTENCIA GENETICA / Root knot nematodes / ROOTS / SECUENCIA NUCLEOTIDICA / SEGREGACION / SEGREGATION / Sequence Homology, Amino Acid / SEQUENCE NUCLEOTIDIQUE / Solanum lycopersicum / Solanum lycopersicum var. lycopersicum / Transcription Factors / Transcription, Genetic / TRANSGENIC PLANTS / YEAST ARTIFICIAL CHROMOSOMES