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Substrate elasticity provides mechanical signals for the expansion of hemopoietic stem and progenitor cells
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Substrate elasticity provides mechanical signals for the expansion of hemopoietic stem and progenitor cells
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Journal Article
Substrate elasticity provides mechanical signals for the expansion of hemopoietic stem and progenitor cells
2010
Overview
Biomechanical forces may be an effective approach for controlling the behavior of stem cells
in vitro
. Holst
et al
. show that the elasticity of a tropoelastin matrix expands hematopoietic stem and progenitor cells.
Surprisingly little is known about the effects of the physical microenvironment on hemopoietic stem and progenitor cells. To explore the physical effects of matrix elasticity on well-characterized primitive hemopoietic cells, we made use of a uniquely elastic biomaterial, tropoelastin. Culturing mouse or human hemopoietic cells on a tropoelastin substrate led to a two- to threefold expansion of undifferentiated cells, including progenitors and mouse stem cells. Treatment with cytokines in the presence of tropoelastin had an additive effect on this expansion. These biological effects required substrate elasticity, as neither truncated nor cross-linked tropoelastin reproduced the phenomenon, and inhibition of mechanotransduction abrogated the effects. Our data suggest that substrate elasticity and tensegrity are important mechanisms influencing hemopoietic stem and progenitor cell subsets and could be exploited to facilitate cell culture.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Cell Proliferation - drug effects
/ Cross-Linking Reagents - metabolism
/ Establishment of new cell lines, improvement of cultural methods, mass cultures
/ Fundamental and applied biological sciences. Psychology
/ Hematopoietic Stem Cells - cytology
/ Hematopoietic Stem Cells - drug effects
/ Hematopoietic Stem Cells - metabolism
/ Humans
/ letter
/ Methods. Procedures. Technologies
/ Mice
/ Mutant Proteins - metabolism
/ Quartz Crystal Microbalance Techniques
/ Signal Transduction - drug effects
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