Asset Details
Do you wish to reserve the book?
Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission
Do you wish to request the book?
Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission
2022
Overview
Plasmodium vivax
is the most widespread human malaria parasite. Due to the presence of extravascular reservoirs and relapsing infections from dormant liver stages,
P. vivax
is particularly difficult to control and eliminate. Experimental research is hampered by the inability to maintain
P. vivax
cultures in vitro, due to its tropism for immature red blood cells (RBCs). Here, we describe a new humanized mice model that can support efficient human erythropoiesis and maintain long-lasting multiplication of inoculated cryopreserved
P. vivax
parasites and their sexual differentiation, including in bone marrow. Mature gametocytes were transmitted to
Anopheles
mosquitoes, which led to the formation of salivary gland sporozoites. Importantly, blood-stage
P. vivax
parasites were maintained after the secondary transfer of fresh or frozen infected bone marrow cells to naïve chimeras. This model provides a unique tool for investigating, in vivo, the biology of intraerythrocytic
P. vivax
.
Plasmodium vivax is the most widespread human malaria parasite but due to its tropism for immature red blood cells in vitro culturing and studies are difficult. Here, LuizaBatista et al. generate a humanized mouse supporting human erythropoiesis and allowing asexual and sexual development of P. vivax in bone marrow and peripheral blood, as well as transmission to mosquitoes.
