Asset Details
Do you wish to reserve the book?
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
Do you wish to request the book?
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
2013
Overview
The genome of human cells is edited using the bacterial RNA-guided Cas9 endonuclease.
We employ the CRISPR-Cas system of
Streptococcus pyogenes
as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33%.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical Engineering/Biotechnology
/ DNA
/ DNA, Recombinant - metabolism
/ Genetic Engineering - methods
/ Histocompatibility Antigens - genetics
/ Histocompatibility Antigens - metabolism
/ Humans
/ Luminescent Proteins - genetics
/ Luminescent Proteins - metabolism
/ Methods
/ Receptors, CCR5 - metabolism
/ Restriction-modification system
/ RNA
/ Streptococcus pyogenes - enzymology
