Journal Article

Low phosphate activates STOP1-ALMT1 to rapidly inhibit root cell elongation

Pellequer, Jean-Luc, Delannoy, Etienne, David, Pascale, Balzergue, Coline, CEA (APTTOX021401, APTTOX021403), Müller, Jens, Becuwe-Linka, Noëlle, Thibaud, Marie-Christine, Brouchoud, Corinne, Biochimie et Physiologie Moléculaire des Plantes (BPMP) ; Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ANR-11-RSNR-0005,DEMETERRES,Développement de Méthodes bio-et Eco-Technologiques pour la Remédiation Raisonnée des Effluents et des Sols en appui à une stratégie de réhabilitation agricole post-accidentelle, ANR-09-BLAN-0118,CHEMIGENA,Génétique d'une voie limitant la croissance racinaire d'Arabidopsis en réponse à la carence phosphatée, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM) ; Aix Marseille Université (AMU)-Centre Nation

2017

Overview

Environmental cues profoundly modulate cell proliferation and cell elongation to inform and direct plant growth and development. External phosphate (Pi) limitation inhibits primary root growth in many plant species. However, the underlying Pi sensory mechanisms are unknown. Here we genetically uncouple two Pi sensing pathways in the root apex of Arabidopsis thaliana. First, the rapid inhibition of cell elongation in the transition zone is controlled by transcription factor STOP1, by its direct target, ALMT1, encoding a malate channel, and by ferroxidase LPR1, which together mediate Fe and peroxidase-dependent cell wall stiffening. Second, during the subsequent slow inhibition of cell proliferation in the apical meristem, which is mediated by LPR1-dependent, but largely STOP1–ALMT1-independent, Fe and callose accumulate in the stem cell niche, leading to meristem reduction. Our work uncovers STOP1 and ALMT1 as a signalling pathway of low Pi availability and exuded malate as an unexpected apoplastic inhibitor of root cell wall expansion.

• Publisher: Nature Publishing Group,CCSD,Nature Publishing Group UK,Nature Portfolio
• Subject: 14/19 / 14/34 / 38 / 38/77 / 631/449 / 631/449/2661/2665 / 64 / 96 / Arabidopsis - genetics / Arabidopsis - metabolism / Arabidopsis Proteins - genetics / Arabidopsis Proteins - metabolism / Botanics / Cell Enlargement / Cell growth / Cell Wall - genetics / Cell Wall - metabolism / Gene Expression Regulation, Plant / Genes / Humanities and Social Sciences / Iron - metabolism / Irritable bowel syndrome / Life Sciences / Malates - metabolism / Meristem - cytology / Meristem - genetics / Meristem - metabolism / multidisciplinary / Mutation / Organic Anion Transporters - genetics / Organic Anion Transporters - metabolism / Oxidoreductases - genetics / Oxidoreductases - metabolism / Peroxidase - genetics / Peroxidase - metabolism / Phosphates - metabolism / Plant growth / Plant Roots - cytology / Plant Roots - genetics / Plant Roots - metabolism / Plant sciences / Plant species / Plants, Genetically Modified / Science / Science (multidisciplinary) / Signal Transduction - genetics / Stem cells / Transcription factors / Transcription Factors - genetics / Transcription Factors - metabolism / Transition zone / Vegetal Biology
• ISBN: 0004012788000