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Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis
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Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis
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Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis
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Journal Article
Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis
2015
Overview
Hyaluronic acid (HA), a natural high molecular weight polysaccharide, is produced by Streptococcus zooepidemicus. However, Streptococcus has several drawbacks including its potential to produce exotoxins, so there is demand for an alternative HA source. Here, a recombinant HA biosynthesis operon, as well as the HA biosynthesis operon of S. zooepidemicus were introduced into L. lactis using the nisin-controlled expression system, respectively. HA was successfully synthesized by recombinant L. lactis. Furthermore, overexpression of the endogenous enzymes directing the synthesis of precursor sugars was effective at increasing HA production, and increasing the supply of UDP-activated monosaccharide donors aided synthesis of monodisperse HA polysaccharides. Besides GRAS host strain (L. lactis) and NICE system, the selecting marker (lacF gene) of the recombinant strain is also food grade. Therefore, HA produced by recombinant L. lactis overcomes the problems associated with Streptococcus and provides a source of food-grading HA appropriate for widespread biotechnological applications.
Publisher
Springer-Verlag,Oxford University Press,Springer Berlin Heidelberg
Subject
/ Analysis
/ Bioenergy/Biofuels/Biochemicals
/ Biomedical and Life Sciences
/ DNA, Bacterial - isolation & purification
/ E coli
/ Enzymes
/ Food
/ Glucose
/ Hyaluronic Acid - biosynthesis
/ Lactococcus lactis - genetics
/ Lactococcus lactis - metabolism
/ Lactose
/ operon
/ Plasmids
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
/ Streptococcus equi - genetics
/ Streptococcus equi subsp. zooepidemicus
/ Studies
/ sugars
/ Yeast
