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Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
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Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
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Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
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Journal Article
Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
2017
Overview
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into
SlIAA9
—a key gene controlling parthenocarpy—with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit—a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38/22
/ 38/23
/ 42/70
/ 45/22
/ 45/23
/ Breeding
/ CRISPR
/ CRISPR-Cas Systems - genetics
/ Genomes
/ Humanities and Social Sciences
/ Mutation
/ Plants, Genetically Modified
/ RNA, Guide, CRISPR-Cas Systems - genetics
/ Science
/ Solanum lycopersicum - genetics
/ Solanum lycopersicum - growth & development
/ Tomatoes
