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Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

by Pérez-Ramos, Adrián , López, Paloma , Requena, Teresa , García-Cayuela, Tomás , Mohedano, M. Luz , Gaiser, Rogier A

in Amino Acid Sequence / Analysis / Animal sciences / Bacteria / Bacterial Proteins - genetics / Bacterial Proteins - metabolism / bacteriocins / Bacteriocins - genetics / Bacteriocins - metabolism / Biochemistry / Bioinformatics / Biomedical and Life Sciences / Biotechnology / Biotechnology Methods / cloning / coculture / Coculture Techniques / controlled gene-expression / DNA, Bacterial - genetics / E coli / Enzymes / Escherichia coli / Gene expression / Genetic Engineering / Genetic Vectors - genetics / Gram-positive bacteria / industrial microbiology / Inorganic Chemistry / lactic-acid bacteria / Lactobacillus acidophilus / Lactobacillus acidophilus - genetics / Lactobacillus acidophilus - metabolism / lactococcus-lactis / Life Sciences / Microbiology / Molecular Sequence Data / Natural environment / plasmid / Promoter Regions, Genetic / Proteins / Reproducibility of Results / Streptococcus infections / Streptococcus pneumoniae / Studies / Sucrose / transcription (genetics)

2015

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Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

by Pérez-Ramos, Adrián , López, Paloma , Requena, Teresa , García-Cayuela, Tomás , Mohedano, M. Luz , Gaiser, Rogier A

2015

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Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

by Pérez-Ramos, Adrián , López, Paloma , Requena, Teresa , García-Cayuela, Tomás , Mohedano, M. Luz , Gaiser, Rogier A

2015

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Journal Article

Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

Pérez-Ramos, Adrián,

López, Paloma,

Requena, Teresa,

García-Cayuela, Tomás,

Mohedano, M. Luz,

Gaiser, Rogier A

2015

Overview

Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene expression in Lactobacillus. This vector is also able to replicate in Streptococcus pneumoniae and Escherichia coli. The usage of pRCR as a promoter probe was validated in Lactobacillus acidophilus by characterizing the regulation of lactacin B expression. The results show that the regulation is exerted at the transcriptional level, with lbaB gene expression being specifically induced by co-culture of the L. acidophilus bacteriocin producer and the S. thermophilus STY-31 inducer bacterium.

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Publisher

Springer-Verlag,Oxford University Press,Springer Berlin Heidelberg

Subject

/ Bacterial Proteins - genetics

/ Bacterial Proteins - metabolism

/ bacteriocins