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Production of genome-edited Daphnia for heavy metal detection by fluorescence
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Production of genome-edited Daphnia for heavy metal detection by fluorescence
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Journal Article
Production of genome-edited Daphnia for heavy metal detection by fluorescence
2020
Overview
Aquatic heavy metal pollution is a growing concern. To facilitate heavy metal monitoring in water, we developed transgenic
Daphnia
that are highly sensitive to heavy metals and respond to them rapidly. Metallothionein A, which was a metal response gene, and its promoter region was obtained from
Daphnia magna
. A chimeric gene fusing the promoter region with a green fluorescent protein (GFP) gene was integrated into
D. magna
using the TALEN technique and transgenic
Daphnia
named
D. magna
MetalloG were produced. When
D. magna
MetalloG was exposed to heavy metal solutions for 1 h, GFP expression was induced only in their midgut and hepatopancreas. The lowest concentrations of heavy metals that activated GFP expression were 1.2 µM Zn
2+
, 130 nM Cu
2+
, and 70 nM Cd
2+
. Heavy metal exposure for 24 h could lower the thresholds even further.
D. magna
MetalloG facilitates aqueous heavy metal detection and might enhance water quality monitoring.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Animals
/ Animals, Genetically Modified - genetics
/ Animals, Genetically Modified - metabolism
/ Copper
/ Daphnia
/ Environmental Monitoring - methods
/ Genetic Engineering - methods
/ Genomes
/ Humanities and Social Sciences
/ Metallothionein - metabolism
/ Midgut
/ Science
/ Water Pollutants, Chemical - analysis
/ Water Pollutants, Chemical - metabolism
/ Zinc
