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Endogenous insensitivity to the Orco agonist VUAA1 reveals novel olfactory receptor complex properties in the specialist fly Mayetiola destructor
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Endogenous insensitivity to the Orco agonist VUAA1 reveals novel olfactory receptor complex properties in the specialist fly Mayetiola destructor
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Endogenous insensitivity to the Orco agonist VUAA1 reveals novel olfactory receptor complex properties in the specialist fly Mayetiola destructor
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Journal Article
Endogenous insensitivity to the Orco agonist VUAA1 reveals novel olfactory receptor complex properties in the specialist fly Mayetiola destructor
2018
Overview
Insect olfactory receptors are routinely expressed in heterologous systems for functional characterisation. It was recently discovered that the essential olfactory receptor co-receptor (Orco) of the Hessian fly,
Mayetiola destructor
(Mdes), does not respond to the agonist VUAA1, which activates Orco in all other insects analysed to date. Here, using a mutagenesis-based approach we identified three residues in MdesOrco, located in different transmembrane helices as supported by 3D modelling, that confer sensitivity to VUAA1. Reciprocal mutations in
Drosophila melanogaster
(Dmel) and the noctuid moth
Agrotis segetum
(Aseg) Orcos diminish sensitivity of these proteins to VUAA1. Additionally, mutating these residues in DmelOrco and AsegOrco compromised odourant receptor (OR) dependent ligand-induced Orco activation. In contrast, both wild-type and VUAA1-sensitive MdesOrco were capable of forming functional receptor complexes when coupled to ORs from all three species, suggesting unique complex properties in
M. destructor
, and that not all olfactory receptor complexes are “created” equal.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 82/80
/ 96
/ 96/34
/ Agonists
/ Animals
/ Biologi
/ Cell Membrane - drug effects
/ Drosophila melanogaster - genetics
/ Drosophila Proteins - antagonists & inhibitors
/ Drosophila Proteins - genetics
/ Humanities and Social Sciences
/ Insects
/ Ligands
/ Mutation
/ Olfactory Receptor Neurons - drug effects
/ Proteins
/ Receptors, Odorant - antagonists & inhibitors
/ Receptors, Odorant - genetics
/ Science
/ Thioglycolates - pharmacology
/ Zoologi
/ Zoology
