Journal Article

Oral bacterial composition associated with lung function and lung inflammation in a community-based Norwegian population

Lin, Huang, Peddada, Shyamal, Gómez Real, Francisco, Svanes, Cecilie, Bertelsen, Randi Jacobsen, Shigdel, Rajesh, Johannessen, Ane, Ringel-Kulka, Tamar

2023

Overview

Background The oral cavity is the gateway to the bacteria community in the lung. Disruption of the symbiotic balance of the oral microbiota has been associated with respiratory diseases. However, little is known about the relationship between oral bacteria and respiratory outcomes in the general population. We aimed to describe the associations between oral bacteria, lung function, and lung inflammation in a community-based population. Methods Oral (gingival) samples were collected concurrently with spirometry tests in 477 adults (47% males, median age 28 years) from the RHINESSA study in Bergen, Norway. Bacterial DNA from the 16S rRNA gene from gingival fluid were sequenced by Illumina ® MiSeq. Lung function was measured using spirometry and measurement of fractional exhaled nitric oxide (FeNO) were performed to examine airway inflammation. Differential abundance analysis was performed using ANCOM-BC, adjusting for weight, education, and smoking. Results The abundance of the genera Clostridiales , Achromobacter, Moraxella , Flavitalea and Helicobacter were significantly different among those with low FEV 1 (< lower limit of normal (LLN)) as compared to normal FEV 1 i.e. ≥ LLN. Twenty-three genera differed in abundance between among those with low FVC < LLN as compared to normal FEV 1  ≥ LLN. The abundance of 27 genera from phyla Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Sacchribacteria differed significantly between elevated FeNO levels (≥ 50 ppb) compared to FeNO ≤ 25 ppb. Conclusion Oral bacterial composition was significantly different for those with low FEV or FVC as compared to those with normal lung function equal to or higher than LLN. Differential bacterial composition was also observed for elevated FeNO levels.

• Publisher: BioMed Central,BioMed Central Ltd,Nature Publishing Group,BMC
• Subject: Abundance / Achromobacter / Age / Analysis / Antibiotics / Asthma / Bacteria / Care and treatment / Chronic obstructive pulmonary disease / Comment / Composition / Forced expiratory volume / Forced vital capacity / Fractional exhaled nitric oxide / Inflammation / Lung function / Lungs / Medicine / Medicine & Public Health / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Nitric oxide / Nucleotide sequence / Oral cavity / Oral microbiome / Pneumology/Respiratory System / Properties / Pulmonary function tests / Questionnaires / Respiratory diseases / Respiratory function / Respiratory tract diseases / rRNA 16S / Sensitivity analysis / Spirometry / Veganism