Journal Article

Circular RNA circTRIM33–12 acts as the sponge of MicroRNA-191 to suppress hepatocellular carcinoma progression

Peng, Rui, Ke, Ai-Wu, Yang, Xuan, Gao, Chao, Fan, Jia, Gao, Dong-Mei, Cai, Jia-Bin, Lu, Jia-Cheng, Zhang, Peng-Fei, Zhang, Chi, Huang, Xiao-Yong, Wei, Chuan-Yuan, Gao, Ping-Ting, Shi, Guo-Ming

2019

Overview

Background Recently, the dysregulation of circular RNA (circRNA) have been shown to have important regulatory roles in cancer development and progression, including hepatocellular carcinoma (HCC). However, the roles of most circRNAs in HCC are still unknown. Methods The expression of circular tripartite motif containing 33–12 (circTRIM33–12) in HCC tissues and cell lines was detected by qRT-PCR. The role of circTRIM33–12 in HCC progression was assessed by western blotting, CCK-8, flow cytometry, transwell and a subcutaneous tumor mouse assays both in vitro and in vivo. In vivo circRNA precipitation, RNA immunoprecipitation, luciferase reporter assays were performed to evaluate the interaction between circTRIM33–12 and miR-191. Results Here, we found that circTRIM33–12, is downregulated in HCC tissues and cell lines. The downregulation of circTRIM33–12 in HCC was significantly correlated with malignant characteristics and served as an independent risk factor for the overall survival (OS) and recurrence-free survival (RFS) of patients with HCC after surgery. The reduced expression of circTRIM33–12 in HCC cells increases tumor proliferation, migration, invasion and immune evasion. Mechanistically, we demonstrated that circTRIM33–12 upregulated TET1 expression by sponging miR-191, resulting in significantly reduced 5-hydroxymethylcytosine (5hmC) levels in HCC cells. Conclusions These results reveal the important role of circTRIM33–12 in the proliferation, migration, invasion and immune evasion abilities of HCC cells and provide a new perspective on circRNAs in HCC progression.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Biomedical and Life Sciences / Biomedicine / Cancer / Cancer Research / Carcinoma / Carcinoma, Hepatocellular - genetics / Carcinoma, Hepatocellular - pathology / Cell cycle / Cell growth / Cell Line, Tumor / Cell migration / Cell Proliferation / Cholecystokinin / Circular RNA / Deoxyribonucleic acid / Development and progression / Disease Progression / DNA / DNA methylation / Down-Regulation / Female / Flow cytometry / Gene expression / Gene Expression Regulation, Neoplastic / Genetic aspects / Hep G2 Cells / Hepatocellular carcinoma / Histone methylation / Humans / Immunoglobulins / Immunoprecipitation / Journalists / Liver cancer / Liver Neoplasms - genetics / Liver Neoplasms - pathology / Luciferase / Male / Medical prognosis / Metastasis / Methylation / MicroRNA / MicroRNAs / MicroRNAs - genetics / miRNA / Mixed Function Oxygenases - genetics / Neoplasm Invasiveness / Neoplasm Transplantation / Oncology / Plasmids / Polymerase chain reaction / Prognosis / Proto-Oncogene Proteins - genetics / Risk factors / RNA / RNA, Circular - genetics / Statistical analysis / Surgery / Survival Analysis / TET1 / Tumorigenesis / Tumors / Western blotting