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Prediction of enzymatic pathways by integrative pathway mapping
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Journal Article
Prediction of enzymatic pathways by integrative pathway mapping
2018
Overview
The functions of most proteins are yet to be determined. The function of an enzyme is often defined by its interacting partners, including its substrate and product, and its role in larger metabolic networks. Here, we describe a computational method that predicts the functions of orphan enzymes by organizing them into a linear metabolic pathway. Given candidate enzyme and metabolite pathway members, this aim is achieved by finding those pathways that satisfy structural and network restraints implied by varied input information, including that from virtual screening, chemoinformatics, genomic context analysis, and ligand -binding experiments. We demonstrate this integrative pathway mapping method by predicting the L-gulonate catabolic pathway in Haemophilus influenzae Rd KW20. The prediction was subsequently validated experimentally by enzymology, crystallography, and metabolomics. Integrative pathway mapping by satisfaction of structural and network restraints is extensible to molecular networks in general and thus formally bridges the gap between structural biology and systems biology.
Publisher
eLife Science Publications, Ltd,eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd
Subject
/ Carbon
/ Computational and Systems Biology
/ Computational Biology - methods
/ Enzymes
/ Genomes
/ Haemophilus influenzae - genetics
/ Haemophilus influenzae - metabolism
/ Kinases
/ l-gulonate catabolic pathway
/ Labeling
/ Ligands
/ Metabolic Networks and Pathways - genetics
/ Proteins
/ Structural Biology and Molecular Biophysics
