Journal Article

Antibiotics-induced perturbations in gut microbial diversity influence metabolic phenotypes in a murine model of high-fat diet-induced obesity

Wen, Beibei, Li, Yinhua, Liu, Dongmin, Liu, Zhonghua, Lin, Haiyan, Luo, Yong, Li, Juan, Huang, Jianan, Zhu, Kun

2019

Overview

Gut microbiota play a key role in the regulation of obesity and associated metabolic disorders. To study the relationship between them, antibiotics have been widely used to generate pseudo-germ-free rodents as control models. However, it is not clear whether antibiotics impact an animal’s metabolic phenotype. Therefore, the effect of antibiotics-induced gut microbial perturbations on metabolic phenotypes in high-fat diet (HFD) fed mice was investigated. The results showed that antibiotics perturbed gut microbial composition and structure. Community diversity and richness were reduced, and the phyla Firmicutes / Bacteroidetes (F/B) ratio was decreased by antibiotics. Visualization of Unifrac distance data using principal component analysis (PCA) and unweighted pair-group method with arithmetic mean (UPGAM) demonstrated that fecal samples of HFD-fed mice separated from those of chow diet (CD) fed mice. Fecal samples from antibiotics-treated and non-treated mice were clustered into two different microbial populations. Moreover, antibiotics suppressed HFD-induced metabolic features, including body weight gain (BWG), liver weight (LW), epididymal fat weight (EFW), and serum levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), fasting blood glucose (FBG), and insulin (INS) significantly ( P < 0 .05). Lachnospiraceae , Ruminiclostridium and Helicobacter , biomarkers of mouse gut microbiota before treatment by antibiotics, were positively correlated with obesity phenotypes significantly ( P < 0 .05) and were decreased by (92.95 ± 5.09) %, (97.73 ± 2.09) % and (99.48 ± 0.21) % respectively after 30 days of treatment by antibiotics. However, Bacteroidia were enriched in HFD-fed antibiotics-treated mice and were negatively correlated with obesity phenotypes significantly ( P < 0 .05). We suggested that the antibiotics-induced depletion of Lachnospiraceae , Ruminiclostridium , and Helicobacter , and the decrease in F/B ratio in gut microbiota played a role in the prevention of HFD-induced obesity in mice.

• Publisher: Springer Berlin Heidelberg,Springer,Springer Nature B.V
• Subject: Alanine / Alanine transaminase / Animal models / Antibiotics / Applied Genetics and Molecular Biotechnology / Bacteroidia / Biomarkers / Biomedical and Life Sciences / Biotechnology / blood glucose / blood serum / Body weight / body weight changes / Body weight gain / Causes of / Cholesterol / Complications and side effects / Data processing / Density / Depletion / Diet / Distribution / epididymis / fasting / Feces / Germfree / Gut microbiota / Helicobacter / High density lipoprotein / high density lipoprotein cholesterol / High fat diet / Insulin / Intestinal microflora / intestinal microorganisms / Lachnospiraceae / Life Sciences / Liver / Low density lipoprotein / low density lipoprotein cholesterol / metabolic diseases / Metabolic disorders / Metabolism / Mice / Microbial Genetics and Genomics / Microbiology / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Obesity / Perturbation / phenotype / Phenotypes / Physiological aspects / principal component analysis / Principal components analysis / Rodents / Serum levels