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Discovery and identification of potential anti-melanogenic active constituents of Bletilla striata by zebrafish model and molecular docking
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Discovery and identification of potential anti-melanogenic active constituents of Bletilla striata by zebrafish model and molecular docking
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Journal Article
Discovery and identification of potential anti-melanogenic active constituents of Bletilla striata by zebrafish model and molecular docking
2022
Overview
Background
Bletilla striata
is the main medicine of many skin whitening classic formulas in traditional Chinese medicine (TCM) and is widely used in cosmetic industry recently. However, its active ingredients are still unclear and its fibrous roots are not used effectively. The aim of the present study is to discover and identify its potential anti-melanogenic active constituents by zebrafish model and molecular docking.
Methods
The antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2′-azino-bis-(3-ethylbenthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay
.
The anti-melanogenic activity was assessed by tyrosinase inhibitory activity in vitro and melanin inhibitory in zebrafish. The chemical profiles were performed by ultra-high-performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Meanwhile, the potential anti-melanogenic active constituents were temporary identified by molecular docking.
Results
The 95% ethanol extract of
B. striata
fibrous roots (EFB) possessed the strongest DPPH, ABTS, FRAP and tyrosinase inhibitory activities, with IC
50
5.94 mg/L, 11.69 mg/L, 6.92 mmol FeSO
4
/g, and 58.92 mg/L, respectively. In addition, EFB and 95% ethanol extract of
B. striata
tuber (ETB) significantly reduced the melanin synthesis of zebrafish embryos in a dose-dependent manner. 39 chemical compositions, including 24 stilbenoids were tentatively identified from EFB and ETB. Molecular docking indicated that there were 83 (including 60 stilbenoids) and 85 (including 70 stilbenoids) compounds exhibited stronger binding affinities toward tyrosinase and adenylate cyclase.
Conclusion
The present findings supported the rationale for the use of EFB and ETB as natural skin-whitening agents in pharmaceutical and cosmetic industries.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Acids
/ Animals
/ China
/ Complementary & Alternative Medicine
/ Embryos
/ Ethanol
/ Formulae, receipts, prescriptions
/ High performance liquid chromatography
/ industry
/ Medicine
/ Medicine, Chinese Traditional - methods
/ Melanin
/ Melanins - antagonists & inhibitors
/ Melanoma
/ Molecular Docking Simulation
/ Monophenol Monooxygenase - antagonists & inhibitors
/ Oriental traditional medicine
/ Plant Extracts - pharmacology
/ Polysaccharides - pharmacology
/ Radicals
/ Reagents
/ Roots
/ Skin
/ Skin Lightening Preparations - chemistry
/ Skin Lightening Preparations - pharmacology
/ Traditional Chinese medicine
