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High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions
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High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions
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High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions
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Journal Article
High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions
2023
Overview
Background
Pediocin PA-1 is a bacteriocin of recognized value with applications in food bio-preservation and the medical sector for the prevention of infection. To date, industrial manufacturing of pediocin PA-1 is limited by high cost and low-performance. The recent establishment of the biotechnological workhorse
Corynebacterium glutamicum
as recombinant host for pediocin PA-1 synthesis displays a promising starting point towards more efficient production.
Results
Here, we optimized the fermentative production process. Following successful simplification of the production medium, we carefully investigated the impact of dissolved oxygen, pH value, and the presence of bivalent calcium ions on pediocin production. It turned out that the formation of the peptide was strongly supported by an acidic pH of 5.7 and microaerobic conditions at a dissolved oxygen level of 2.5%. Furthermore, elevated levels of CaCl
2
boosted production. The IPTG-inducible producer
C
. glutamicum
CR099
pXMJ19
P
tac
pedACD
Cg
provided 66 mg L
−1
of pediocin PA-1 in a two-phase batch process using the optimized set-up. In addition, the novel constitutive strain
P
tuf
pedACD
Cg
allowed successful production without the need for IPTG.
Conclusions
The achieved pediocin titer surpasses previous efforts in various microbes up to almost seven-fold, providing a valuable step to further explore and develop this important bacteriocin. In addition to its high biosynthetic performance
C. glutamicum
proved to be highly robust under the demanding producing conditions, suggesting its further use as host for bacteriocin production.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
