Journal Article

Three-dimensional printed hydroxyapatite bone tissue engineering scaffold with antibacterial and osteogenic ability

Shaohong, Wu, Jian, Cao, Haipeng, Gao, Jinlong, Li, Zhongxing, Liu, Limin, Zhang, Yuanzheng, Wang

2021

Overview

The development of an effective scaffold for bone defect repair is an urgent clinical need. However, it is challenging to design a scaffold with efficient osteoinduction and antimicrobial activity for regeneration of bone defect. In this study, we successfully prepared a hydroxyapatite (HA) porous scaffold with a surface-specific binding of peptides during osteoinduction and antimicrobial activity using a three-dimensional (3D) printing technology. The HA binding domain (HABD) was introduced to the C-terminal of bone morphogenetic protein 2 mimetic peptide (BMP2-MP) and antimicrobial peptide of PSI10. The binding capability results showed that BMP2-MP and PSI10-containing HABD were firmly bound to the surface of HA scaffolds. After BMP2-MP and PSI10 were bound to the scaffold surface, no negative effect was observed on cell proliferation and adhesion. The gene expression and protein translation levels of type I collagen (COL-I), osteocalcin (OCN) and Runx2 have been significantly improved in the BMP2-MP/HABP group. The level of alkaline phosphatase significantly increased in the BMP2-MP/HABP group. The inhibition zone test against Staphylococcus aureus and Escherichia coli BL21 prove that the PSI10/HABP@HA scaffold has strong antibacterial ability than another group. These findings suggest that 3D-printed HA scaffolds with efficient osteoinduction and antimicrobial activity represent a promising biomaterial for bone defect reconstruction.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: 3D printed / Alkaline phosphatase / Antibacterial / Antiinfectives and antibacterials / Antimicrobial activity / Antimicrobial agents / Antimicrobial peptides / Applied Microbiology / Binding / Biological products / Biological Techniques / Biomaterials / Biomedical Engineering and Bioengineering / Biomedical materials / Biotechnology / Bone biomaterials / Bone growth / Bone morphogenetic protein 2 / Bone morphogenetic proteins / Bone scaffold / Cbfa-1 protein / Cell proliferation / Collagen / Collagen (type I) / E coli / Engineering / Environmental Engineering/Biotechnology / Gene expression / Genetic translation / Growth factors / Hydroxyapatite / Mechanical properties / Methicillin / Nucleic Acid Chemistry / Osteocalcin / Osteoinduction / Peptides / Phosphatases / Political aspects / Polymers / Polypeptides / Proteins / Regeneration / Scaffolds / Scanning electron microscopy / Software / Staphylococcus infections / Three dimensional printing / Tissue engineering