Asset Details
Do you wish to reserve the book?
Genome-wide association study of leaf-related traits in tea plant in Guizhou based on genotyping-by-sequencing
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Genome-wide association study of leaf-related traits in tea plant in Guizhou based on genotyping-by-sequencing
Do you wish to request the book?
Genome-wide association study of leaf-related traits in tea plant in Guizhou based on genotyping-by-sequencing
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Genome-wide association study of leaf-related traits in tea plant in Guizhou based on genotyping-by-sequencing
2023
Overview
Background
Studying the genetic characteristics of tea plant (
Camellia
spp.) leaf traits is essential for improving yield and quality through breeding and selection. Guizhou Plateau, an important part of the original center of tea plants, has rich genetic resources. However, few studies have explored the associations between tea plant leaf traits and single nucleotide polymorphism (SNP) markers in Guizhou.
Results
In this study, we used the genotyping-by-sequencing (GBS) method to identify 100,829 SNP markers from 338 accessions of tea germplasm in Guizhou Plateau, a region with rich genetic resources. We assessed population structure based on high-quality SNPs, constructed phylogenetic relationships, and performed genome-wide association studies (GWASs). Four inferred pure groups (G-I, G-II, G-III, and G-IV) and one inferred admixture group (G-V), were identified by a population structure analysis, and verified by principal component analyses and phylogenetic analyses. Through GWAS, we identified six candidate genes associated with four leaf traits, including mature leaf size, texture, color and shape. Specifically, two candidate genes, located on chromosomes 1 and 9, were significantly associated with mature leaf size, while two genes, located on chromosomes 8 and 11, were significantly associated with mature leaf texture. Additionally, two candidate genes, located on chromosomes 1 and 2 were identified as being associated with mature leaf color and mature leaf shape, respectively. We verified the expression level of two candidate genes was verified using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and designed a derived cleaved amplified polymorphism (dCAPS) marker that co-segregated with mature leaf size, which could be used for marker-assisted selection (MAS) breeding in
Camellia sinensis
.
Conclusions
In the present study, by using GWAS approaches with the 338 tea accessions population in Guizhou, we revealed a list of SNPs markers and candidate genes that were significantly associated with four leaf traits. This work provides theoretical and practical basis for the genetic breeding of related traits in tea plant leaves.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Analysis
/ Biomedical and Life Sciences
/ Breeding
/ Camellia sinensis - genetics
/ China
/ Chromosome Mapping - methods
/ Color
/ Genes
/ Genome-wide association studies
/ Genome-Wide Association Study
/ Genome-wide association study (GWAS)
/ Genomes
/ Genotype
/ Genotyping-by-sequencing (GBS)
/ Growth
/ Leaves
/ Methods
/ Plants
/ Polymorphism, Single Nucleotide - genetics
/ quantitative polymerase chain reaction
/ Single nucleotide polymorphism (SNP)
/ Single nucleotide polymorphisms
/ Single-nucleotide polymorphism
/ Tea
/ Texture
