Journal Article

Stress- and ubiquitylation-dependent phase separation of the proteasome

Inada, Toshifumi, Tsuchiya, Hikaru, Kaiho, Ai, Ohtake, Fumiaki, Baumeister, Wolfgang, Arai, Naoko, Ikeuchi, Ken, Endo, Akinori, Murata, Shigeo, Fernández-Busnadiego, Rubén, Tanaka, Keiji, Guo, Qiang, Yasuda, Sayaka, Saeki, Yasushi

2020

Overview

The proteasome is a major proteolytic machine that regulates cellular proteostasis through selective degradation of ubiquitylated proteins 1 , 2 . A number of ubiquitin-related molecules have recently been found to be involved in the regulation of biomolecular condensates or membraneless organelles, which arise by liquid–liquid phase separation of specific biomolecules, including stress granules, nuclear speckles and autophagosomes 3 – 8 , but it remains unclear whether the proteasome also participates in such regulation. Here we reveal that proteasome-containing nuclear foci form under acute hyperosmotic stress. These foci are transient structures that contain ubiquitylated proteins, p97 (also known as valosin-containing protein (VCP)) and multiple proteasome-interacting proteins, which collectively constitute a proteolytic centre. The major substrates for degradation by these foci were ribosomal proteins that failed to properly assemble. Notably, the proteasome foci exhibited properties of liquid droplets. RAD23B, a substrate-shuttling factor for the proteasome, and ubiquitylated proteins were necessary for formation of proteasome foci. In mechanistic terms, a liquid–liquid phase separation was triggered by multivalent interactions of two ubiquitin-associated domains of RAD23B and ubiquitin chains consisting of four or more ubiquitin molecules. Collectively, our results suggest that ubiquitin-chain-dependent phase separation induces the formation of a nuclear proteolytic compartment that promotes proteasomal degradation. Hyperosmotic stress leads to a phase separation of the proteasome, triggered by interactions between RAD23B and ubiquitylated proteins, which bring together p97 and proteasome-associated proteins into nuclear proteolytic foci.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 14/19 / 14/28 / 631/80/474/2085 / 631/80/474/582 / 82/58 / Biomolecules / Cell Line / Cell Nucleus - metabolism / Chains / Degradation / DNA Repair Enzymes - metabolism / DNA-Binding Proteins - metabolism / Humanities and Social Sciences / Humans / Liquid phases / Methods / multidisciplinary / Observations / Organelles / Osmotic Pressure / Phase separation / Polyethylene glycol / Polyubiquitin - metabolism / Proteasome Endopeptidase Complex - chemistry / Proteasome Endopeptidase Complex - metabolism / Proteasomes / Proteins / Proteolysis / Proteostasis / Regulation / Ribosomal proteins / Ribosomal Proteins - metabolism / Science / Science (multidisciplinary) / Scientific imaging / Separation (Technology) / Stress, Physiological / Substrates / Sucrose / Ubiquitin / Ubiquitin-proteasome system / Ubiquitin-Protein Ligases - metabolism / Ubiquitination / Valosin Containing Protein - metabolism / Valosin-containing protein