Asset Details
Do you wish to reserve the book?
An improved cyan fluorescent protein variant useful for FRET
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
An improved cyan fluorescent protein variant useful for FRET
Do you wish to request the book?
An improved cyan fluorescent protein variant useful for FRET
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
An improved cyan fluorescent protein variant useful for FRET
2004
Overview
Many genetically encoded biosensors use Förster resonance energy transfer (FRET) between fluorescent proteins to report biochemical phenomena in living cells. Most commonly, the enhanced cyan fluorescent protein (ECFP) is used as the donor fluorophore, coupled with one of several yellow fluorescent protein (YFP) variants as the acceptor. ECFP is used despite several spectroscopic disadvantages, namely a low quantum yield, a low extinction coefficient and a fluorescence lifetime that is best fit by a double exponential. To improve the characteristics of ECFP for FRET measurements, we used a site-directed mutagenesis approach to overcome these disadvantages. The resulting variant, which we named Cerulean (ECFP/S72A/Y145A/H148D), has a greatly improved quantum yield, a higher extinction coefficient and a fluorescence lifetime that is best fit by a single exponential. Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation.
Publisher
Nature Publishing Group US,Nature,Nature Publishing Group
Subject
/ Animals
/ Bacterial Proteins - chemistry
/ Biological and medical sciences
/ Biomedical Engineering/Biotechnology
/ Electrophoresis, Polyacrylamide Gel
/ Fluorescence resonance energy transfer
/ Fluorescence Resonance Energy Transfer - methods
/ Fundamental and applied biological sciences. Psychology
/ Indicators and Reagents - pharmacology
/ letter
/ Light
/ Luminescent Proteins - chemistry
/ Luminescent Proteins - pharmacology
/ Mutation
/ Proteins
