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A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue
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A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue
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Journal Article
A combined human case of Dirofilaria ursi infection in dorsal subcutaneous tissue and Anisakis simplex sensu stricto (s.s.) infection in ventral subcutaneous tissue
2017
Overview
Background
Dirofilaria ursi
is a filarial nematode that parasitizes the subcutaneous tissues of the American black bear (
Ursus americanus
) and Japanese black bear (
Ursus thiabetanus japonicus
).
D
.
ursi
that has parasitized black bears has the potential to subsequently infect humans. In addition, extra-gastrointestinal anisakiasis is less common in Japan.
Case presentation
We report a case of ventral subcutaneous anisakiasis and dorsal subcutaneous dirofilariasis that was acquired in Fukushima, in the northern part of Japan. The patient was an 83-year-old Japanese female, and subcutaneous parasitic granulomas were present on her left abdomen (near the navel) and left scapula. A pathological examination of the surgically dissected tissue sections from each region demonstrated eosinophilic granulomas containing different species of parasites. To enable the morphological and molecular identification of these parasites, DNA was extracted from paraffin-embedded sections using DEXPAT reagent, and the cytochrome oxidase 2 (COX2), internal transcribed spacer 1 (ITS1), 5.8S and ITS2 regions of the
Anisakis
larvae, and the 5S rRNA region of the male
Dirofilaria
were sequenced. The PCR products were examined and compared with DNA databases. Molecular analysis of the COX2 and 5S rRNA sequences of each worm revealed that the nematode found in the ventral region belonged to
Anisakis simplex
sensu stricto (s.s.) and the male
Dirofilaria
found in the dorsal region was classified as
D. ursi
.
Conclusion
The present case showed a combined human case of
D. ursi
and
A. simplex
s.s. infections in subcutaneous tissues. The results of this study will contribute to the identification of unknown parasites in histological sections.
Publisher
BioMed Central,Springer Nature B.V,BMC
