Journal Article

Recombinant L. lactis vaccine LL-plSAM-WAE targeting four virulence factors provides mucosal immunity against H. pylori infection

Wang, Xuequan, Qu, Yuliang, Liu, Jing, Zhou, Wenmiao, Zhang, Zhen, Zhang, Furui, Chen, Jiale, Ni, Linhan, Guo, Le, Luo, Xuegang, Liu, Kunmei

2024

Overview

Background Helicobacter pylori ( H. pylori ) causes chronic gastric disease. An efficient oral vaccine would be mucosa-targeted and offer defense against colonization of invasive infection in the digestive system. Proteolytic enzymes and acidic environment in the gastrointestinal tract (GT) can, however, reduce the effectiveness of oral vaccinations. For the creation of an edible vaccine, L. lactis has been proposed as a means of delivering vaccine antigens. Results We developed a plSAM (pNZ8148-SAM) that expresses a multiepitope vaccine antigen SAM-WAE containing Urease, HpaA, HSP60, and NAP extracellularly (named LL-plSAM-WAE) to increase the efficacy of oral vaccinations. We then investigated the immunogenicity of LL-plSAM-WAE in Balb/c mice. Mice that received LL-plSAM-WAE or SAM-WAE with adjuvant showed increased levels of antibodies against H. pylori , including IgG and sIgA, and resulted in significant reductions in H. pylori colonization. Furthermore, we show that SAM-WAE and LL-plSAM-WAE improved the capacity to target the vaccine to M cells. Conclusions These findings suggest that recombinant L. lactis could be a promising oral mucosa vaccination for preventing H. pylori infection.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Analysis / Antibodies / Antigens / Applied Microbiology / Biotechnology / Care and treatment / Chemistry / Chemistry and Materials Science / Chronic infection / Colonization / Complications and side effects / Diagnosis / Digestive system / DNA vaccines / Dosage and administration / Enzymes / Enzymology / Gastrointestinal diseases / Gastrointestinal system / Gastrointestinal tract / Genetic Engineering / Helicobacter infections / Helicobacter pylori / Hsp60 protein / Immune response / Immunogenicity / Immunoglobulin G / Immunology / Infections / Investigations / Lactic acid bacteria / Lactococcus lactis / Ligands / M cell-targeting / M cells / Microbial Genetics and Genomics / Microbiology / Mucosal immune response / Mucosal immunity / Proteins / Proteolysis / Proteolytic enzymes / Risk factors / Urease / Vaccine delivery system / Vaccines / Virulence / Virulence (Microbiology) / Virulence factors