Asset Details
Do you wish to reserve the book?
Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model
Do you wish to request the book?
Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Surfactant Protein D modulates allergen particle uptake and inflammatory response in a human epithelial airway model
2012
Overview
Background
Allergen-containing subpollen particles (SPP) are released from whole plant pollen upon contact with water or even high humidity. Because of their size SPP can preferentially reach the lower airways where they come into contact with surfactant protein (SP)-D. The aim of the present study was to investigate the influence of SP-D in a complex three-dimensional human epithelial airway model, which simulates the most important barrier functions of the epithelial airway. The uptake of SPP as well as the secretion of pro-inflammatory cytokines was investigated.
Methods
SPP were isolated from timothy grass and subsequently fluorescently labeled. A human epithelial airway model was built by using human Type II-pneumocyte like cells (A549 cells), human monocyte derived macrophages as well as human monocyte derived dendritic cells. The epithelial cell model was incubated with SPP in the presence and absence of surfactant protein D. Particle uptake was evaluated by confocal microscopy and advanced computer-controlled analysis. Finally, human primary CD4
+
T-Cells were added to the epithelial airway model and soluble mediators were measured by enzyme linked immunosorbent assay or bead array.
Results
SPP were taken up by epithelial cells, macrophages, and dendritic cells. This uptake coincided with secretion of pro-inflammatory cytokines and chemokines. SP-D modulated the uptake of SPP in a cell type specific way (e.g. increased number of macrophages and epithelial cells, which participated in allergen particle uptake) and led to a decreased secretion of pro-inflammatory cytokines.
Conclusion
These results display a possible mechanism of how SP-D can modulate the inflammatory response to inhaled allergen.
Publisher
BioMed Central,BioMed Central Ltd,Nature Publishing Group,BMC
Subject
/ Allergy
/ Alveolar Epithelial Cells - immunology
/ Alveolar Epithelial Cells - metabolism
/ Analysis
/ Animals
/ Asthma
/ CD4-Positive T-Lymphocytes - immunology
/ CD4-Positive T-Lymphocytes - metabolism
/ Dendritic Cells - immunology
/ Dendritic Cells - metabolism
/ Enzymes
/ Epithelial Cells - immunology
/ Epithelial Cells - metabolism
/ Hostages
/ Humans
/ Medicine
/ Pneumology/Respiratory System
/ Pollen
/ Proteins
/ Pulmonary Surfactant-Associated Protein D - immunology
/ Pulmonary Surfactant-Associated Protein D - metabolism
/ Rats
/ SP-D
/ SPP
/ T cells
