Journal Article

Effect of Anti-ApoA-I Antibody-Coating of Stents on Neointima Formation in a Rabbit Balloon-Injury Model

Strang, Aart C., Tak, Paul P., Koole, Leo H., Rotmans, Joris I., Bisoendial, Radjesh J., de Winter, Robbert J., Knetsch, Menno L. W., de Vries, Carlie J. M., Stroes, Erik S. G., van der Wal, Allard C.

2015

Overview

Since high-density lipoprotein (HDL) has pro-endothelial and anti-thrombotic effects, a HDL recruiting stent may prevent restenosis. In the present study we address the functional characteristics of an apolipoprotein A-I (ApoA-I) antibody coating in vitro. Subsequently, we tested its biological performance applied on stents in vivo in rabbits. The impact of anti ApoA-I- versus apoB-antibody coated stainless steel discs were evaluated in vitro for endothelial cell adhesion, thrombin generation and platelet adhesion. In vivo, response to injury in the iliac artery of New Zealand white rabbits was used as read out comparing apoA-I-coated versus bare metal stents. ApoA-I antibody coated metal discs showed increased endothelial cell adhesion and proliferation and decreased thrombin generation and platelet adhesion, compared to control discs. In vivo, no difference was observed between ApoA-I and BMS stents in lumen stenosis (23.3±13.8% versus 23.3±11.3%, p=0.77) or intima surface area (0.81±0.62 mm2 vs 0.84±0.55 mm2, p=0.85). Immunohistochemistry also revealed no differences in cell proliferation, fibrin deposition, inflammation and endothelialization. ApoA-I antibody coating has potent pro-endothelial and anti-thrombotic effects in vitro, but failed to enhance stent performance in a balloon injury rabbit model in vivo.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adhesion / Adhesion tests / Animals / Antibodies / Antibodies, Monoclonal - immunology / Antibodies, Monoclonal - pharmacology / Apolipoprotein A / Apolipoprotein A-I / Apolipoprotein A-I - immunology / Apolipoproteins / Atherosclerosis / Biomedical engineering / Biomedical materials / Blood platelets / Cell adhesion / Cell adhesion & migration / Cell Adhesion - drug effects / Cell proliferation / Cell Proliferation - drug effects / Cloning / Coating effects / Coatings / Disease Models, Animal / Endothelial cells / Endothelial Cells - cytology / Endothelial Cells - drug effects / Female / Fibrin / High density lipoprotein / Homeostasis - drug effects / Humans / Immunohistochemistry / Implants / In vivo methods and tests / Injuries / Low density lipoprotein / Medical research / Metals / Microvessels - cytology / Neointima - metabolism / Neointima - pathology / Neointima - physiopathology / Neointima - prevention & control / Platelet Adhesiveness - drug effects / Platelets / Rabbits / Restenosis / Stainless steel / Stenosis / Stents / Surface Properties / Surgical implants / Thrombin / Thrombin - biosynthesis / Thromboembolism / Veins & arteries