Journal Article

Isolation and Identification of Dominant Bacteria from Raw Donkey Milk Produced in a Region of Morocco by QIIME 2 and Evaluation of Their Antibacterial Activity

Quinteiro, Javier, Derdak, Reda, Rodríguez Castro, Jorge, Rey Méndez, Manuel, El Khalfi, Bouchra, Addoum, Boutaina, Sakoui, Souraya, Soukri, Abdelaziz

2021

Overview

Recently, the interest in donkey milk has increased considerably because it proved high nutritive and functional values of their ingredients. Its chemical composition is widely studied, but its microbiota, especially lactic acid bacteria, remains less studied. This study focuses on analyzing, isolating, and identifying lactic acid bacteria and evaluating their capacity to produce biomolecules with antibacterial activity. Among 44 strains identified, 43 are Gram-positive, and most are catalase-negative and cocci-shaped. Five strains were selected to evaluate their antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli. Different induction methods allowed to amplify the antibacterial effects against these pathogenic strains.

• Publisher: Hindawi,John Wiley & Sons, Inc,Wiley
• Subject: Aerococcus - chemistry / Aerococcus - isolation & purification / Aerococcus - metabolism / Analysis / Animals / Anti-Bacterial Agents - pharmacology / Antibacterial activity / Antibacterial agents / Antibiotics / Antimicrobial agents / Bacteria / Biomolecules / Catalase / Chemical composition / Chemical contaminants / Contamination / Culture Media, Conditioned - pharmacology / Dairy products / Dairying - methods / E coli / Enterococcus - chemistry / Enterococcus - isolation & purification / Enterococcus - metabolism / Enterococcus faecalis - chemistry / Enterococcus faecalis - isolation & purification / Enterococcus faecalis - metabolism / Equidae / Escherichia coli / Escherichia coli - drug effects / Escherichia coli - growth & development / Escherichia coli - pathogenicity / Ethylenediaminetetraacetic acid / Female / Food Microbiology / Glucose / Lactation - physiology / Lactic acid / Lactic acid bacteria / Leuconostoc mesenteroides - chemistry / Leuconostoc mesenteroides - isolation & purification / Leuconostoc mesenteroides - metabolism / Listeria / Listeria monocytogenes / Listeria monocytogenes - drug effects / Listeria monocytogenes - growth & development / Listeria monocytogenes - pathogenicity / Metabolites / Microbial Sensitivity Tests / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Milk / Milk - microbiology / Morocco / Phenolphthalein / Ribosomal DNA / Staphylococcus aureus / Staphylococcus aureus - drug effects / Staphylococcus aureus - growth & development / Staphylococcus aureus - pathogenicity / Strains (organisms) / Taxonomy