Journal Article

Interferon epsilon is produced in the testis and protects the male reproductive tract against virus infection, inflammation and damage

Hedger, Mark P., Hasan, Hiba, Stanton, Peter, Pilatz, Adrian, Fietz, Daniela, Hertzog, Paul J., Schuppe, Hans-Christian, Gearing, Linden J., Wray-McCann, Georgie, Tate, Michelle D., Fijak, Monika, Sreenivasan, Rajini, Wijayarathna, Rukmali, de Geus, Eveline D., Genovese, Rosemary

2024

Overview

The testis is a reservoir for viruses that can cause persistent infection and adversely affect male reproductive health, an observation commonly attributed to deficiencies in inducible antiviral defence mechanisms. In this study, we demonstrate that interferon-epsilon (IFNε), a type I interferon initially discovered in female reproductive epithelia, is constitutively expressed by meiotic and post-meiotic spermatogenic cells, Leydig cells and macrophages in mouse testes. A similar distribution pattern was observed in human testes. Mice lacking IFNɛ were more susceptible to Zika virus-induced inflammation and damage of the testis and epididymis compared to wild-type mice. Exogenous IFNε treatment reduced the viral infection burden in cultured human testicular cells by inducing interferon-stimulated gene expression, and reducing inflammatory gene expression and cell damage. Treatment was more effective when administered prior to infection. These data indicate a critical role for constitutively-expressed IFNɛ in limiting viral infection and inflammatory damage in the male reproductive tract.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animal research / Animals / Asymptomatic / Bats / Biology and Life Sciences / Biopsy / Care and treatment / Damage / Development and progression / Epididymis / Ethics / Gene expression / Genes / Genital diseases, Male / Genitalia, Male - metabolism / Genitalia, Male - pathology / Genitalia, Male - virology / Genotype & phenotype / Health aspects / Humans / Inflammation / Inflammation - metabolism / Interferon / Interferon Type I - metabolism / Leukocytes / Leydig cells / Macrophages / Male / Males / Medical research / Medicine and Health Sciences / Meiosis / Mice / Mice, Inbred C57BL / Mice, Knockout / Patients / Physiological aspects / Reproductive health / Reproductive system / Sperm / Spermatogenesis / Testes / Testis / Testis - metabolism / Testis - pathology / Testis - virology / Testosterone / Urology / Vector-borne diseases / Viral infections / Virus diseases / Viruses / Zika Virus / Zika Virus Infection - immunology / Zika Virus Infection - virology