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The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer
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The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer
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Journal Article
The long non-coding RNA HOTAIR indicates a poor prognosis and promotes metastasis in non-small cell lung cancer
2013
Overview
Background
The identification of cancer-associated long non-coding RNAs and the investigation of their molecular and biological functions are important for understanding the molecular biology and progression of cancer. HOTAIR (
HOX transcript antisense intergenic RNA
) has been implicated in several cancers; however, its role in non-small cell lung cancer (NSCLC) is unknown. The aim of the present study was to examine the expression pattern of HOTAIR in NSCLC and to evaluate its biological role and clinical significance in tumor progression.
Methods
Expression of HOTAIR was analyzed in 42 NSCLC tissues and four NSCLC cell lines by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Over-expression and RNA interference (RNAi) approaches were used to investigate the biological functions of HOTAIR. The effect of HOTAIR on proliferation was evaluated by MTT and colony formation assays, and cell migration and invasion were evaluated by transwell assays. Tail vein injection of cells was used to study metastasis in nude mice. Protein levels of HOTAIR targets were determined by western blot analysis. Differences between groups were tested for significance using Student’s t-test (two-tailed).
Results
HOTAIR was highly expressed both in NSCLC samples and cell lines compared with corresponding normal counterparts. HOTAIR upregulation was correlated with NSCLC advanced pathological stage and lymph-node metastasis. Moreover, patients with high levels of HOTAIR expression had a relatively poor prognosis. Inhibition of HOTAIR by RNAi decreased the migration and invasion of NSCLC cells
in vitro
and impeded cell metastasis
in vivo
. HOXA5 levels were affected by HOTAIR knockdown or over-expression
in vitro
.
Conclusions
Our findings indicate that HOTAIR is significantly up-regulated in NSCLC tissues, and regulates NSCLC cell invasion and metastasis, partially via the down-regulation of HOXA5. Thus, HOTAIR may represent a new marker of poor prognosis and is a potential therapeutic target for NSCLC intervention.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V
Subject
/ Biology
/ Biomedical and Life Sciences
/ Carcinoma, Non-Small-Cell Lung - genetics
/ Carcinoma, Non-Small-Cell Lung - mortality
/ Carcinoma, Non-Small-Cell Lung - pathology
/ Disease
/ Female
/ Gene Expression Regulation, Neoplastic
/ Genomes
/ Health Promotion and Disease Prevention
/ Homeodomain Proteins - metabolism
/ Humans
/ Male
/ Matrix Metalloproteinase 2 - metabolism
/ Matrix Metalloproteinase 9 - metabolism
/ Oncology
/ Proteins
