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Absence of Wolbachia endobacteria in the human parasitic nematode Dracunculus medinensis and two related Dracunculus species infecting wildlife
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Absence of Wolbachia endobacteria in the human parasitic nematode Dracunculus medinensis and two related Dracunculus species infecting wildlife
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Absence of Wolbachia endobacteria in the human parasitic nematode Dracunculus medinensis and two related Dracunculus species infecting wildlife
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Journal Article
Absence of Wolbachia endobacteria in the human parasitic nematode Dracunculus medinensis and two related Dracunculus species infecting wildlife
2014
Overview
Background
Wolbachia
endosymbionts are a proven target for control of human disease caused by filarial nematodes. However, little is known about the occurrence of
Wolbachia
in taxa closely related to the superfamily Filarioidea. Our study addressed the status of
Wolbachia
presence in members of the superfamily Dracunculoidea by screening the human parasite
Dracunculus medinensis
and related species from wildlife for
Wolbachia
.
Findings
D. medinensis
,
D. lutrae
and
D. insignis
specimens were all negative for
Wolbachia
colonization by PCR screening for the
Wolbachia ftsZ
, 16S rRNA and
Wolbachia
surface protein (
wsp
) sequences. The quality and purity of the DNA preparations was confirmed by amplification of nematode 18S rRNA and cytochrome c oxidase subunit I sequences. Furthermore,
Wolbachia
endobacteria were not detected by whole mount fluorescence staining, or by immunohistochemistry using a
Wolbachia
-specific antiserum. In contrast, positive control
Brugia malayi
worms were shown to harbour
Wolbachia
by PCR, fluorescence staining and immunohistochemistry.
Conclusions
Three examined species of
Dracunculus
showed no evidence of
Wolbachia
endobacteria. This supports that members of the superfamily Dracunculoidea are free of
Wolbachia
. Within the order Spirurida, these endosymbionts appear restricted to the Filarioidea.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
