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Embryonic Stem Cells-loaded Gelatin Microcryogels Slow Progression of Chronic Kidney Disease
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Embryonic Stem Cells-loaded Gelatin Microcryogels Slow Progression of Chronic Kidney Disease
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Journal Article
Embryonic Stem Cells-loaded Gelatin Microcryogels Slow Progression of Chronic Kidney Disease
2016
Overview
Background： Chronic kidney disease （CKD） has become a public health problem.New interventions to slow or prevent disease progression are urgently needed.In this setting, cell therapies associated with regenerative effects are attracting increasing interest.We evaluated the effect of embryonic stem cells （ESCs） on the progression of CKD.Methods： Adult male Sprague-Dawley rats were subjected to 5/6 nephrectomy.We used pedicled greater omentum flaps packing ESC-loaded gelatin microcryogets （GMs） on the 5/6 nephrectomized kidney.The viability of ESCs within the GMs was detected using in vio two-photon fluorescence confocal imaging.Rats were sacrificed after 12 weeks.Renal injury was evaluated using serum creatinine, urea nitrogen, 24 h protein, renal pathology, and tubular injury score results.Structural damage was evaluated by periodic acid-Schiff and Masson trichrome staining.Results： In vitro, ESCs could be automatically loaded into the GMs.Uniform cell distribution, good cell attachment, and viability were achieved from day 1 to 7 in vitro.After 12 weeks, in the pedicled greater omentum flaps packing ESC-loaded GMs on 5/6 nephrectomized rats group, the plasma urea nitrogen levels were 26% lower than in the right nephrectomy group, glomerulosclerosis index was 62% lower and tubular injury index was 40% lower than in the 5/6 nephrectomized rats group without GMs.Conclusions： In a rat model of established CKD, we demonstrated that the pedicled greater omentum flaps packing ESC-loaded GMs on the 5/6 nephrectomized kidney have a long-lasting therapeutic rescue function, as shown by the decreased progression of CKD and reduced glomerular injury.
Publisher
Wolters Kluwer - Medknow Publications,Medknow Publications and Media Pvt. Ltd,Lippincott Williams & Wilkins Ovid Technologies,Department of Nephrology%Department of Biotechnology, School of Chemistry and Life Science, Changchun University of Technology, Changchun, Jilin 130012, China,Medknow Publications & Media Pvt Ltd,Wolters Kluwer
Subject
/ Chronic Renal Insufficiency; Embryonic Stem Cells; Gelatin Microcryogels; Regenerative Medicine
/ Cryogels
/ Embryonic Stem Cells - transplantation
/ Gelatin - administration & dosage
/ Male
/ Mice
/ Original
/ Rats
/ Renal Insufficiency, Chronic - pathology
/ Renal Insufficiency, Chronic - therapy
/ Rodents
/ SD大鼠
/ 公共卫生问题
/ 慢性
/ 明胶
/ 肾脏疾病
/ 肾脏病
/ 胚胎干细胞
/ 自动加载
