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α-Fucosidases with different substrate specificities from two species of Fusarium
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Journal Article
α-Fucosidases with different substrate specificities from two species of Fusarium
2013
Overview
Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of
Fusarium oxysporum
strain 0685 grown on
l
-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on
p-
nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative α-fucosidase gene (
FgFCO1
) from
Fusarium graminearum
was expressed in
Pichia pastoris
. FgFCO1 was ∼1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 α-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes.
Trichoderma reesei
and most species of
Aspergillus
lack genes for either GH29 subfamily.
Publisher
Springer-Verlag,Springer,Springer Nature B.V
Subject
/ alpha-L-Fucosidase - genetics
/ alpha-L-Fucosidase - isolation & purification
/ alpha-L-Fucosidase - metabolism
/ Analysis
/ Biology
/ Biomedical and Life Sciences
/ Biotechnologically Relevant Enzymes and Proteins
/ Fungi
/ Fusarium
/ Genes
/ genetics
/ Genomes
/ Genomics
/ Mammals
/ Microbial Genetics and Genomics
/ Proteins
/ Sequence Homology, Amino Acid
/ Studies
