Journal Article

Prenatal environmental exposures associated with sex differences in childhood obesity and neurodevelopment

Mendez, Tavis L., Urquiza, Jose, Wright, John, Cáceres, Alejandro, Slama, Remy, Went, Hannah, Chatzi, Leda, Smith, Ryan, Gutzkow, Kristine Bjerve, Andrusaityte, Sandra, Casas, Maribel, Carreras-Gallo, Natàlia, Lepeule, Johanna, Bustamante, Mariona, González, Juan R., Maitre, Léa, Dwaraka, Varun B., Vrijheid, Martine, Stratakis, Nikos, Nieuwenhuijsen, Mark, Carracedo, Ángel, Grazuleviciene, Regina, Yang, Tiffany, Thomsen, Cathrine

2023

Overview

Background Obesity and neurodevelopmental delay are complex traits that often co-occur and differ between boys and girls. Prenatal exposures are believed to influence children’s obesity, but it is unknown whether exposures of pregnant mothers can confer a different risk of obesity between sexes, and whether they can affect neurodevelopment. Methods We analyzed data from 1044 children from the HELIX project, comprising 93 exposures during pregnancy, and clinical, neuropsychological, and methylation data during childhood (5–11 years). Using exposome-wide interaction analyses, we identified prenatal exposures with the highest sexual dimorphism in obesity risk, which were used to create a multiexposure profile. We applied causal random forest to classify individuals into two environments: E1 and E0. E1 consists of a combination of exposure levels where girls have significantly less risk of obesity than boys, as compared to E0, which consists of the remaining combination of exposure levels. We investigated whether the association between sex and neurodevelopmental delay also differed between E0 and E1. We used methylation data to perform an epigenome-wide association study between the environments to see the effect of belonging to E1 or E0 at the molecular level. Results We observed that E1 was defined by the combination of low dairy consumption, non-smokers’ cotinine levels in blood, low facility richness, and the presence of green spaces during pregnancy (OR interaction  = 0.070, P  = 2.59 × 10 −5 ). E1 was also associated with a lower risk of neurodevelopmental delay in girls, based on neuropsychological tests of non-verbal intelligence (OR interaction  = 0.42, P  = 0.047) and working memory (OR interaction  = 0.31, P  = 0.02). In line with this, several neurodevelopmental functions were enriched in significant differentially methylated probes between E1 and E0. Conclusions The risk of obesity can be different for boys and girls in certain prenatal environments. We identified an environment combining four exposure levels that protect girls from obesity and neurodevelopment delay. The combination of single exposures into multiexposure profiles using causal inference can help determine populations at risk.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Age / Biomarkers / Biomedicine / Body mass index / Causal inference / Child / Child Development / Childhood / Childhood obesity / Children / Children & youth / Clinical outcomes / Cognition in children / Cognitive ability / Cotinine / Delay / Demographic aspects / Disease / DNA methylation / Environmental aspects / Environmental Exposure - adverse effects / Environmental Exposure - analysis / Exposure / Female / fetal and childhood outcomes / Gender differences / Girls / Health aspects / Humans / Life Sciences / Male / Maternal factors during pregnancy influencing maternal / Medicine / Medicine & Public Health / Memory / Methylation / Neurodevelopment / Obesity / Obesity in children / Outdoor air quality / Pediatric Obesity / Pregnancy / Prenatal environment / Prenatal experience / Prenatal exposure / Prenatal Exposure Delayed Effects - epidemiology / Prenatal influences / Public health / Research Article / Risk / Risk factors / Santé publique et épidémiologie / Sex Characteristics / Sex differences / Sexual dimorphism / Short term memory / Urine