Journal Article

Performance of Ag-ELISA in the diagnosis of Taenia solium cysticercosis in naturally infected pigs in Tanzania

Trevisan, Chiara, Lightowlers, Marshall W., Kabululu, Mwemezi L., Colston, Angela, Cordel, Claudia, Ngowi, Helena A., Mkupasi, Ernatus M., Mlangwa, James E. D., Johansen, Maria V., Braae, Uffe C.

2020

Overview

Background Taenia solium is a zoonotic parasite responsible for neurocysticercosis—a major cause of late-onset acquired epilepsy in humans. Lack of affordable, specific and sensitive diagnostic tools hampers control of the parasite. This study assessed the performance of an antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) in the diagnosis of viable T. solium cysticercosis in naturally infected slaughter-age pigs in an endemic area in Tanzania. Methods A total of 350 pigs were bled before they were slaughtered and their carcases examined. Serum was analyzed for circulating antigens by using a monoclonal antibody-based B158/B60 Ag-ELISA. Each carcase was examined for the presence of Taenia hydatigena cysticerci and half carcase musculature together with the whole brain, head muscles, tongue, heart and diaphragm were sliced with fine cuts (< 0.5 cm) to reveal and enumerate T. solium cysticerci. Half carcase dissection can detect at least 84% of infected pigs. Prevalence and their 95% confidence intervals (CI) were calculated in Stata 12. Sensitivity, specificity, predictive values and likelihood ratios were determined. Results Twenty–nine pigs (8.3%, 95% CI: 5.6–11.7%) had viable T. solium cysticerci while 11 pigs had T. hydatigena cysticerci (3.1%, 95% CI: 1.6–5.5%). No co-infection was observed. Sixty-eight pigs (19.4%, 95% CI: 15.4–20%) tested positive on Ag-ELISA; of these, 24 had T. solium cysticerci and 7 had T. hydatigena cysticerci. Sensitivity and specificity were determined to be 82.7% and 86.3%, respectively. Positive and negative predictive values were 35.2% and 98.2%, respectively. Likelihood ratios for positive and negative Ag-ELISA test results were 6.0 and 0.2, respectively. There was a significant positive correlation between the titre of circulating antigens and intensity of T. solium cysticerci ( r (348)  = 0.63, P  < 0.001). Conclusions The Ag-ELISA test characteristics reported in this study indicate that the test is more reliable in ruling out T. solium cysticercosis in pigs, than in confirming it. Hence, a negative result will almost certainly indicate that a pig has no infection, but a positive result should always be interpreted with caution. Estimates of T. solium prevalence based on Ag-ELISA results should, therefore, be adjusted for test performance characteristics and occurrence of T. hydatigena .

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Ag-ELISA / Animals / Antibodies / Antibodies, Helminth - blood / antigen detection / Antigens / Biomedical and Life Sciences / Biomedicine / blood serum / brain / Brain slice preparation / Cardiac muscle / Confidence intervals / cysticerci / Cysticercosis / Cysticercosis - diagnosis / Cysticercosis - transmission / Cysticercosis - veterinary / Diagnosis / Diagnostic software / Diagnostic systems / Diaphragm / Diaphragm (anatomy) / Dissection / ELISA / Entomology / Enzyme-linked immunosorbent assay / Enzyme-Linked Immunosorbent Assay - methods / Enzyme-Linked Immunosorbent Assay - veterinary / Enzymes / Epilepsy / head / Health aspects / Heart / Hogs / Immunologic tests / Infections / Infectious Diseases / Laboratories / Methods / mixed infection / Monoclonal antibodies / Muscles / Neurocysticercosis - transmission / Neurocysticercosis - veterinary / Parasites / Parasitology / Pigs / Probability / Ratios / Sensitivity / Serologic Tests - methods / Serologic Tests - veterinary / Serum / Specificity / swine / Swine - parasitology / Swine diseases / Swine Diseases - diagnosis / Swine Diseases - parasitology / Taenia hydatigena / Taenia solium / Taenia solium - immunology / Taenia solium - isolation & purification / Taeniasis / Tanzania / Tanzania - epidemiology / tongue / Tropical Medicine / Veterinary Medicine/Veterinary Science / Virology / Zoonoses - diagnosis / Zoonoses - parasitology