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RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
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RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
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Journal Article
RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO
2002
Overview
The
RAD6
pathway is central to post-replicative DNA repair in eukaryotic cells; however, the machinery and its regulation remain poorly understood. Two principal elements of this pathway are the ubiquitin-conjugating enzymes RAD6 and the MMS2–UBC13 heterodimer, which are recruited to chromatin by the RING-finger proteins RAD18 and RAD5, respectively. Here we show that UBC9, a small ubiquitin-related modifier (SUMO)-conjugating enzyme, is also affiliated with this pathway and that proliferating cell nuclear antigen (PCNA)—a DNA-polymerase sliding clamp involved in DNA synthesis and repair—is a substrate. PCNA is mono-ubiquitinated through RAD6 and RAD18, modified by lysine-63-linked multi-ubiquitination—which additionally requires MMS2, UBC13 and RAD5—and is conjugated to SUMO by UBC9. All three modifications affect the same lysine residue of PCNA, suggesting that they label PCNA for alternative functions. We demonstrate that these modifications differentially affect resistance to DNA damage, and that damage-induced PCNA ubiquitination is elementary for DNA repair and occurs at the same conserved residue in yeast and humans.
Publisher
Nature Publishing Group UK,Nature Publishing,Nature Publishing Group
Subject
Biological and medical sciences
/ Conserved Sequence - genetics
/ DNA
/ DNA-Binding Proteins - metabolism
/ Enzymes
/ Fundamental and applied biological sciences. Psychology
/ Humanities and Social Sciences
/ Humans
/ Molecular and cellular biology
/ Mutation
/ Proliferating Cell Nuclear Antigen - genetics
/ Proliferating Cell Nuclear Antigen - metabolism
/ Saccharomyces cerevisiae - genetics
/ Saccharomyces cerevisiae - metabolism
/ Saccharomyces cerevisiae Proteins - genetics
/ Saccharomyces cerevisiae Proteins - metabolism
/ Science
/ Small Ubiquitin-Related Modifier Proteins - metabolism
/ Ubiquitin-Conjugating Enzymes
/ Yeasts
