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A single-cell survey of Drosophila blood

by Cho, Bumsik , Hu, Yanhui , Steinbaugh, Michael J , Tattikota, Sudhir Gopal , Comjean, Aram , Nam, Jin-Wu , Perrimon, Norbert , Liu, Yifang , Barrera, Victor , Hung, Ruei-Jiun , Li, Fangge , Dervis, Franz , Shim, Jiwon , Ho Sui, Shannan , Yoon, Sang-Ho

in Animals / blood / Blood cells / Cell Communication / Cell cycle / Cell Lineage / Datasets / Developmental Biology / Drosophila / Drosophila melanogaster - genetics / Drosophila melanogaster - immunology / Drosophila melanogaster - metabolism / Drosophila melanogaster - parasitology / Drosophila Proteins - metabolism / Eggs / Fibroblast growth factors / Fibroblast Growth Factors - metabolism / Gene expression / Genes / Genes, Insect / Genetic research / Hemocytes / Hemocytes - cytology / Hemocytes - immunology / Hemocytes - metabolism / Host-Parasite Interactions / Immune response / Immunity / Immunology and Inflammation / Inflammation / Insects / Larva - genetics / Larva - immunology / Larva - metabolism / Larva - parasitology / Larvae / Novels / Parasitoids / Plasmatocytes / Ribonucleic acid / RNA / RNA sequencing / RNA-Seq / scRNA-seq / Signal Transduction / Single-Cell Analysis / Surveys / Transcription, Genetic / Transcriptome / wasp infestation / Wasps / wounding

2020

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2020

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2020

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Journal Article

A single-cell survey of Drosophila blood

Cho, Bumsik,

Hu, Yanhui,

Steinbaugh, Michael J,

Tattikota, Sudhir Gopal,

Comjean, Aram,

Nam, Jin-Wu,

Perrimon, Norbert,

Liu, Yifang,

Barrera, Victor,

Hung, Ruei-Jiun,

Li, Fangge,

Dervis, Franz,

Shim, Jiwon,

Ho Sui, Shannan,

Yoon, Sang-Ho

2020

Overview

Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand branchless and receptor breathless, respectively. We demonstrate that these FGF components are required for mediating effective immune responses against parasitoid wasp eggs, highlighting a novel role for FGF signaling in inter-hemocyte crosstalk. Our scRNA-seq analysis reveals the diversity of hemocytes and provides a rich resource of gene expression profiles for a systems-level understanding of their functions.

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Publisher

eLife Science Publications, Ltd,eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd

Subject

/ blood