Journal Article

Molecular Epidemiology of Staphylococcus aureus among Patients with Skin and Soft Tissue Infections in Two Chinese Hospitals

Song, Zhen, Han, Li-Zhong, Gu, Fei-Fei, Ni, Yu-Xing, Dong, De-Ping, Chen, Ye, Guo, Xiao-Kui

2016

Overview

Background: Staphylococcus aureus is one of the predominant causes of skin and soft tissue infections (SSTIs), but limited data were available regarding the characterization of S. aureus from SSTIs patients in Jiangsu Province in China. We aimed to investigate the molecular epidemiology of S. aureus among SSTIs patients in two hospitals of Jiangsu Province. Methods: Sixty-two patients with SSTIs from two Chinese hospitals in Jiangsu Province were enrolled in this study, and 62 S. aureus isolates were collected from February 2014 to January 2015. S. aureus isolates were characterized by antimicrobial susceptibility testing, toxin gene detection, and molecular typing with sequence type, Staphylococcus protein A gene type, accessory gene regulator (agr) group, and Staphylococcal cassette chromosome mec t ype. Results: Sixteen (25.8%) methicillin-resistant S. aureus (MRSA) isolates were detected, and there was no isolate found resistant to vancomycin, teicoplanin, sulfamethoxazole-trimethoprim, and linezolid. The sei was the toxin gene most frequently found, and no lukS/F-PV-positive isolates were detected among the SSTIs' patients. Molecular analysis revealed that ST398 (10/62, 16.1%; 2 MRSA and 8 methicillin-susceptible S. aureus) to be the dominant clone, followed by ST5 (8/62, 12.9%) and ST7 (8/62, 12.9%). Conclusions: The livestock ST398 was the most common clone among patients with S. aureus SSTIs in Jiangsu Province, China. Surveillance and further studies on the important livestock ST398 clone in human infections are necessarily requested.

• Publisher: Wolters Kluwer - Medknow Publications,Medknow Publications and Media Pvt. Ltd,Lippincott Williams & Wilkins Ovid Technologies,Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China%Laboratory, Haian Hospital, Nantong University, Nantong, Jiangsu 226600, China%Faculty of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China%Department of Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China,Medknow Publications & Media Pvt Ltd,Wolters Kluwer
• Subject: Adolescent / Adult / Aged / Aged, 80 and over / Anti-Bacterial Agents - pharmacology / Antibiotics / Child / Child, Preschool / China / Cloning / Complications and side effects / Conflicts of interest / Epidemiology / Female / Hospitals / Humans / Infant / Infectious skin diseases / Laboratories / Linezolid - pharmacology / Livestock / Livestock; Molecular Epidemiology; Skin and Soft Tissue Infections; ST398; Staphylococcus aureus / Male / Methicillin-Resistant Staphylococcus aureus - drug effects / Methicillin-Resistant Staphylococcus aureus - isolation & purification / Microbial Sensitivity Tests / Middle Aged / Molecular Epidemiology / Original / Pathogenesis / Patients / Penicillin / Risk factors / Soft Tissue Infections - microbiology / Staphylococcal Infections - microbiology / Staphylococcal Skin Infections - microbiology / Staphylococcus aureus - drug effects / Staphylococcus aureus - isolation & purification / Staphylococcus aureus infections / Staphylococcus infections / Teicoplanin - pharmacology / Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology / Vancomycin - pharmacology / Young Adult