Journal Article

Hydrogen-rich Water Exerting a Protective Effect on Ovarian Reserve Function in a Mouse Model of Immune Premature Ovarian Failure Induced by Zona Pellucida 3

Ma, Yan-Min, Yin, Cheng-Hong, He, Xin, Wang, Shu-Yu, Jia, Chan-Wei, Wang, Tong

2016

Overview

Background: Premature ovarian failure (POF) is a disease that affects female fertility but has few effective treatments. Ovarian reserve function plays an important role in female fertility. Recent studies have reported that hydrogen can protect male fertility. Therefore, we explored the potential protective effect of hydrogen-rich water on ovarian reserve function through a mouse immune POF model. Methods: To set up immune POF model, fifty female BALB/c mice were randomly divided into four groups: Control (mice consumed normal water, n = 10), hydrogen (mice consumed hydrogen-rich water, n = 10), model (mice were immunized with zona pellucida glycoprotein 3 [ZP3] and consumed normal water, n = 15), and model-hydrogen (mice were immunized with ZP3 and consumed hydrogen-rich water, n = 15) groups. After 5 weeks, mice were sacrificed. Serum anti-Müllerian hormone (AMH) levels, granulosa cell (GC) apoptotic index (AI), B-cell leukemia/lymphoma 2 (Bcl-2), and BCL2-associated X protein (Bax) expression were examined. Analyses were performed using SPSS 17.0 (SPSS Inc., Chicago, IL, USA) software. Results: Immune POF model, model group exhibited markedly reduced serum AMH levels compared with those of the control group (5.41 ± 0.91 ng/ml vs. 16.23 ± 1.97 ng/ml, P = 0.033) and the hydrogen group (19.65 ± 7.82 ng/ml, P = 0.006). The model-hydrogen group displayed significantly higher AMH concentrations compared with that of the model group (15.03 ± 2.75 ng/ml vs. 5.41 ± 0.91 ng/ml, P = 0.021). The GC AI was significantly higher in the model group (21.30 ± 1.74%) than those in the control (7.06 ± 0.27%), hydrogen (5.17 ± 0.41%), and model-hydrogen groups (11.24 ± 0.58%) (all P < 0.001). The GC AI was significantly higher in the model-hydrogen group compared with that of the hydrogen group (11.24 ± 0.58% vs. 5.17 ± 0.41%, P = 0.021). Compared with those of the model group, ovarian tissue Bcl-2 levels increased (2.18 ± 0.30 vs. 3.01 ± 0.33, P = 0.045) and the Bax/Bcl-2 ratio decreased in the model-hydrogen group. Conclusions: Hydrogen-rich water may improve serum AMH levels and reduce ovarian GC apoptosis in a mouse immune POF model induced by ZP3.

• Publisher: Wolters Kluwer - Medknow Publications,Medknow Publications and Media Pvt. Ltd,Lippincott Williams & Wilkins Ovid Technologies,Reproductive Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China,Medknow Publications & Media Pvt Ltd,Wolters Kluwer
• Subject: Age / Analysis / Animals / Anti-Mullerian Hormone - blood / Anti-Müllerian Hormone; Apoptotic Index; Granulosa Cells; Hydrogen-rich Water; Immune Premature Ovarian Failure; Ovarian Reserve Function / Apoptosis / Apoptosis - drug effects / bcl-2-Associated X Protein - metabolism / Female / Female infertility / Glycoproteins / Granulosa Cells - cytology / Hydrogen - chemistry / Hydrogen - pharmacology / Infertility / Laboratory animals / Medical research / Mice / Mice, Inbred BALB C / Original / Ovarian Reserve - drug effects / Ovarian Reserve - physiology / Ovaries / Ovary - drug effects / Ovary - metabolism / Primary Ovarian Insufficiency - blood / Primary Ovarian Insufficiency - metabolism / Primary Ovarian Insufficiency - prevention & control / Proteins / Proto-Oncogene Proteins c-bcl-2 - metabolism / Rodents / Studies / Water - administration & dosage / Water - chemistry / Water - pharmacology / Zona Pellucida - drug effects / Zona Pellucida - physiology