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Optimization, purification and characterization of laccase from a new endophytic Trichoderma harzianum AUMC14897 isolated from Opuntia ficus-indica and its applications in dye decolorization and wastewater treatment
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Optimization, purification and characterization of laccase from a new endophytic Trichoderma harzianum AUMC14897 isolated from Opuntia ficus-indica and its applications in dye decolorization and wastewater treatment
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Optimization, purification and characterization of laccase from a new endophytic Trichoderma harzianum AUMC14897 isolated from Opuntia ficus-indica and its applications in dye decolorization and wastewater treatment
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Journal Article
Optimization, purification and characterization of laccase from a new endophytic Trichoderma harzianum AUMC14897 isolated from Opuntia ficus-indica and its applications in dye decolorization and wastewater treatment
2024
Overview
Background
Hazardous synthetic dye wastes have become a growing threat to the environment and public health. Fungal enzymes are eco-friendly, compatible and cost-effective approach for diversity of applications. Therefore, this study aimed to screen, optimize fermentation conditions, and characterize laccase from fungal endophyte with elucidating its ability to decolorize several wastewater dyes.
Results
A new fungal endophyte capable of laccase-producing was firstly isolated from cladodes of
Opuntia ficus
-
indica
and identified as
T. harzianum
AUMC14897 using ITS-rRNA sequencing analysis. Furthermore, the response surface methodology (RSM) was utilized to optimize several fermentation parameters that increase laccase production. The isolated laccase was purified to 13.79-fold. GFC, SDS-PAGE revealed laccase molecular weight at 72 kDa and zymogram analysis elucidated a single band without any isozymes. The peak activity of the pure laccase was detected at 50 °C, pH 4.5, with thermal stability up to 50 °C and half life span for 4 h even after 24 h retained 30% of its activity. The K
m
and V
max
values were 0.1 mM, 22.22 µmol/min and activation energy (E
a
) equal to 5.71 kcal/mol. Furthermore, the purified laccase effectively decolorized various synthetic and real wastewater dyes.
Conclusion
Subsequently, the new endophytic strain produces high laccase activity that possesses a unique characteristic, it could be an appealing candidate for both environmental and industrial applications.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Chemical engineering research
/ Chemistry and Materials Science
/ Coloring Agents - metabolism
/ Endophytes - isolation & purification
/ Fungal Proteins - isolation & purification
/ Fungal Proteins - metabolism
/ Fungi
/ Laccase - isolation & purification
/ Microbial Genetics and Genomics
/ Ovat-statistical laccase optimization
/ Oxidases
/ Sewage
