Journal Article

A High-Density Genetic Map Identifies a Novel Major QTL for Boron Efficiency in Oilseed Rape (Brassica napus L.)

Xu, Fangsen, Shi, Lei, Wang, Xiaohua, Hua, Yingpeng, Zhang, Didi, Zhao, Hua

2014

Overview

Low boron (B) seriously limits the growth of oilseed rape (Brassica napus L.), a high B demand species that is sensitive to low B conditions. Significant genotypic variations in response to B deficiency have been observed among B. napus cultivars. To reveal the genetic basis for B efficiency in B. napus, quantitative trait loci (QTLs) for the plant growth traits, B uptake traits and the B efficiency coefficient (BEC) were analyzed using a doubled haploid (DH) population derived from a cross between a B-efficient parent, Qingyou 10, and a B-inefficient parent, Westar 10. A high-density genetic map was constructed based on single nucleotide polymorphisms (SNPs) assayed using Brassica 60 K Infinium BeadChip Array, simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). The linkage map covered a total length of 2139.5 cM, with 19 linkage groups (LGs) and an average distance of 1.6 cM between adjacent markers. Based on hydroponic evaluation of six B efficiency traits measured in three separate repeated trials, a total of 52 QTLs were identified, accounting for 6.14-46.27% of the phenotypic variation. A major QTL for BEC, qBEC-A3a, was co-located on A3 with other QTLs for plant growth and B uptake traits under low B stress. Using a subset of substitution lines, qBEC-A3a was validated and narrowed down to the interval between CNU384 and BnGMS436. The results of this study provide a novel major locus located on A3 for B efficiency in B. napus that will be suitable for fine mapping and marker-assisted selection breeding for B efficiency in B. napus.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Agricultural production / Arabidopsis / Barley / Biology and Life Sciences / Boron / Boron - metabolism / Boron - pharmacokinetics / Brassica / Brassica napus / Brassica napus - genetics / Brassica napus - growth & development / Brassica napus - metabolism / Breeding / Chromosomes / Crops / Cultivars / Efficiency / Gene loci / Gene mapping / Genetic Linkage / Genetics, Population / Genomes / Genotype & phenotype / Haploidy / Hydroponics / Laboratories / Loci / Marker-assisted selection / Microsatellite Repeats / Oryza / Oryza sativa / Phenotype / Phenotypic variations / Plant breeding / Plant growth / Polymorphism, Single Nucleotide / Population / Quantitative genetics / Quantitative Trait Loci / Rape plants / Rapeseed / Seeds / Simple sequence repeats / Single nucleotide polymorphisms / Single-nucleotide polymorphism / Studies