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Phenotypic effects from the expression of a deregulated AtGAD1 transgene and GABA pathway suppression mutants in maize
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Phenotypic effects from the expression of a deregulated AtGAD1 transgene and GABA pathway suppression mutants in maize
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Phenotypic effects from the expression of a deregulated AtGAD1 transgene and GABA pathway suppression mutants in maize
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Journal Article
Phenotypic effects from the expression of a deregulated AtGAD1 transgene and GABA pathway suppression mutants in maize
2021
Overview
Glutamate decarboxylase (GAD; EC 4.1.1.15) catalyzes the irreversible decarboxylation of glutamate to produce γ-aminobutyric acid (GABA); a ubiquitous non-protein amino acid involved in the regulation of several aspects of plant metabolism and physiology. To study the function of GAD and GABA in maize, we have; 1) introduced native and deregulated forms of
AtGAD1
into maize with the intent of increasing the synthesis of GABA and 2) introduced constructs into maize designed to suppress the activity of several GABA shunt, GABA transport and GABA pathway genes. Maize plants expressing the deregulated AtGAD1 exhibit a severe chlorosis and retarded growth phenotype and have high levels of GABA, and Ca
++
/CaM-independent GAD activity. Plants expressing the suppression constructs for GABA biosynthetic and transport pathway genes had no observable phenotype whereas a knockout of GABA catabolic pathway genes led to growth and developmental defects under standard growth conditions. The implications of this study to our understanding of the action and function of GABA and GAD in crops are discussed.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Analysis
/ Animals
/ Arabidopsis Proteins - genetics
/ Arabidopsis Proteins - metabolism
/ Calcium
/ Corn
/ gamma-Aminobutyric Acid - biosynthesis
/ Gene Expression Regulation, Plant
/ Genes
/ Genetic Complementation Test
/ Genotype
/ Glutamate Decarboxylase - genetics
/ Glutamate Decarboxylase - metabolism
/ Metabolic Networks and Pathways - genetics
/ Mutation
/ Plants, Genetically Modified
/ Proteins
