Journal Article

Evaluation of the detection method by a flotation method using a wire loop for gastrointestinal parasites

Takeshi Hatta, Daikichi Morinaga, Yasutoshi Kido, Isao Teramoto, Kazumi Sasai, Makoto Matsubayashi, Aruto Takano, Akira Kaneko, Hiromitsu Katoh, Shigehiko Uni, Toshimitsu Hatabu, Naotoshi Tsuji

2024

Overview

Infections by gastrointestinal parasites are found in a variety of animals worldwide. For the diagnosis of such infections, the flotation method is commonly used to detect parasitic microorganisms, such as oocysts or eggs, in feces. Instead of adding a flotation solution after the final centrifugation step and using a cover slip to collect the parasites, the method using a wire loop for the recovery of the organisms has been reported as one of alternative methods. However, the recovery rates of microorganisms from the flotation method have not been analysed. In the present study, the utility of a flotation method with the use of a wire loop of 8 mm in diameter (the loop method) was evaluated using different numbers of E. tenella oocysts and Heterakis gallinarum eggs, and chicken fecal samples collected at the farms. Consequently, we found that the oocysts and eggs in tubes could be collected at a ratio of 2.00 to 3.08. Thus, our results indicate that the loop method is a simple and time saving method, implicating the application for the estimated OPG/ EPG (Oocysts/Eggs per gram) of the samples. Graphical ► Utility of a flotation method with the use of a wire loop of 8 mm in diameter was evaluated. ► E. tenella oocysts and Heterakis gallinarum eggs in tubes could be collected at a ratio of 2.00 to 3.08. ► Our results may implicate the application for the estimated OPG/ EPG of the samples as a simple and time saving method.

• Publisher: Wiley,John Wiley & Sons, Inc,John Wiley and Sons Inc
• Subject: Animals / Ascaridoidea - isolation & purification / Case Report / Centrifugation / Chickens / Density / egg / Eggs / Eimeria - isolation & purification / Feces / Feces - parasitology / flotation method / Infections / Intestinal Diseases, Parasitic - diagnosis / Intestinal Diseases, Parasitic - parasitology / Intestinal Diseases, Parasitic - veterinary / Intestinal parasites / loop method / Methods / Microorganisms / oocyst / Oocysts / Oocysts - isolation & purification / Parasite Egg Count - instrumentation / Parasite Egg Count - methods / Parasite Egg Count - veterinary / Parasites / POULTRY / Poultry Diseases - diagnosis / Poultry Diseases - parasitology / SF600-1100 / Sucrose / Veterinary medicine